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Literature summary for 3.2.1.26 extracted from

  • Barbosa, P.M.G.; de Morais, T.P.; de Andrade Silva, C.A.; da Silva Santos, F.R.; Garcia, N.F.L.; Fonseca, G.G.; Leite, R.S.R.; da Paz, M.F.
    Biochemical characterization and evaluation of invertases produced from Saccharomyces cerevisiae CAT-1 and Rhodotorula mucilaginosa for the production of fructooligosaccharides (2018), Prep. Biochem. Biotechnol., 48, 506-513 .
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Rhodotorula mucilaginosa
-
-
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae CAT-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O the hydrolysis by invertases occurs predominantly when sucrose concentration is below 5%. The increase in the concentration of sucrose to levels above 10% results in the highest transferase activity, reaching about 12.6 g/l of nystose Rhodotorula mucilaginosa beta-D-fructose + alpha-D-glucose
-
?
sucrose + H2O the hydrolysis by invertases occurs predominantly when sucrose concentration is below 5%. The increase in the concentration of sucrose to levels above 10% results in the highest transferase activity, reaching about 13.3 g/l of nystose Saccharomyces cerevisiae beta-D-fructose + alpha-D-glucose
-
?
sucrose + H2O the hydrolysis by invertases occurs predominantly when sucrose concentration is below 5%. The increase in the concentration of sucrose to levels above 10% results in the highest transferase activity, reaching about 13.3 g/l of nystose Saccharomyces cerevisiae CAT-1 beta-D-fructose + alpha-D-glucose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
Saccharomyces cerevisiae
70
-
-
Rhodotorula mucilaginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 25°C: about 40% of maximal activity, 60°C: about 65% of maximal activity Saccharomyces cerevisiae
50 80 50°C: about 55% of maximal activity, 80°C: about 85% of maximal activity Rhodotorula mucilaginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Saccharomyces cerevisiae
75
-
stable up to Rhodotorula mucilaginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Rhodotorula mucilaginosa
4.5
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
3 6 pH 3.0: about 90% of maximal activity, pH 6.0: about 50% of maximal activity Rhodotorula mucilaginosa
3.5 6 pH 3.5: about 60% of maximal activity, pH 6.0: about 65% of maximal activity Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 stable Saccharomyces cerevisiae
3 10.5 stable Rhodotorula mucilaginosa