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Literature summary for 3.2.1.26 extracted from
- Bacterial invertases Occurrence, production, biochemical characterization, and significance of transfructosylation (2017), J. Basic Microbiol., 57, 803-813 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Bacillus cereus |  |
| EDTA | - |
Bacillus cereus | |
| EDTA | - |
Limosilactobacillus reuteri | |
| EDTA | 5-10 mM | Thermotoga neapolitana | |
| Fe2+ | - |
Bifidobacterium longum subsp. infantis | |
| Li+ | - |
Bacillus cereus | |
| Mg2+ | - |
Bacillus cereus | |
| Mg2+ | - |
Bifidobacterium longum subsp. infantis | |
| Mn2+ | - |
Bifidobacterium longum subsp. infantis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Limosilactobacillus reuteri | |
| Ca2+ | - |
Limosilactobacillus reuteri | |
| Cd2+ | - |
Bacillus cereus | |
| Cd2+ | - |
Limosilactobacillus reuteri | |
| Cu2+ | - |
Bacillus cereus | |
| Cu2+ | - |
Bifidobacterium longum subsp. infantis | |
| Cu2+ | - |
Corynebacterium glutamicum | |
| Cu2+ | - |
Limosilactobacillus reuteri | |
| CuSO4 | 2 mM | Thermotoga neapolitana | |
| dithiothreitol | - |
Limosilactobacillus reuteri | |
| FeCl2 | 2 mM | Thermotoga neapolitana | |
| Guanidine-HCl | 2-4 M | Thermotoga neapolitana | |
| Hg2+ | - |
Bacillus cereus | |
| Hg2+ | - |
Bifidobacterium longum subsp. infantis | |
| Hg2+ | - |
Limosilactobacillus reuteri | |
| HgCl2 | 2 mM | Thermotoga neapolitana | |
| p-chloromercuribenzoate | - |
Bifidobacterium longum subsp. infantis | |
| Urea | 2 mM | Thermotoga neapolitana | |
| ZnSO4 | 2 mM | Thermotoga neapolitana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.4 | - |
sucrose | pH 6.8, 37°C | Arthrobacter globiformis | |
| 6.6 | - |
sucrose | pH 6.0, 37°C | Limosilactobacillus reuteri | |
| 14 | - |
sucrose | pH and temperature not specified in the publication | Thermotoga neapolitana | |
| 370 | - |
sucrose | pH 6.0, 37°C | Bifidobacterium breve | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Arthrobacter globiformis | - |
- |
| extracellular | - |
Arthrobacter sp. | - |
- |
| extracellular | - |
Levilactobacillus brevis | - |
- |
| extracellular | - |
Zymomonas mobilis | - |
- |
| extracellular | - |
Streptomyces sp. ALKC 8 | - |
- |
| intracellular | - |
Bacillus cereus | 5622 | - |
| intracellular | - |
Corynebacterium glutamicum | 5622 | - |
| intracellular | - |
Paenibacillus macerans | 5622 | - |
| intracellular | - |
Thermotoga neapolitana | 5622 | - |
| intracellular | - |
Bifidobacterium breve | 5622 | - |
| intracellular | - |
Limosilactobacillus reuteri | 5622 | - |
| intracellular | - |
Bifidobacterium longum subsp. infantis | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | 26000 | - |
Bacillus cereus |
| 47000 | - |
- |
Thermotoga neapolitana |
| 58000 | - |
- |
Limosilactobacillus reuteri |
| 60000 | - |
- |
Arthrobacter globiformis |
| 60000 | - |
- |
Bifidobacterium breve |
| 92000 | - |
- |
Corynebacterium glutamicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter globiformis | - |
- |
- |
| Arthrobacter globiformis IFO 3062 | - |
- |
- |
| Arthrobacter sp. | - |
- |
- |
| Arthrobacter sp. 10137 | - |
- |
- |
| Bacillus cereus | - |
- |
- |
| Bacillus cereus TA-11 | - |
- |
- |
| Bifidobacterium breve | - |
- |
- |
| Bifidobacterium breve UCC2003 | - |
- |
- |
| Bifidobacterium longum subsp. infantis | - |
- |
- |
| Bifidobacterium longum subsp. infantis ATCC 15697 | - |
- |
- |
| Corynebacterium glutamicum | - |
- |
- |
| Corynebacterium glutamicum NRRL B-2312 | - |
- |
- |
| Levilactobacillus brevis | - |
- |
- |
| Levilactobacillus brevis Mm-6 | - |
- |
- |
| Limosilactobacillus reuteri | - |
- |
- |
| Limosilactobacillus reuteri CRL 1100 | - |
- |
- |
| Paenibacillus macerans | - |
- |
- |
| Streptomyces sp. ALKC 8 | - |
- |
- |
| Thermotoga neapolitana | - |
- |
- |
| Thermotoga neapolitana DSM 4359T | - |
- |
- |
| Zymomonas mobilis | - |
- |
- |
| Zymomonas mobilis CDBB-B 603 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | - |
Bifidobacterium longum subsp. infantis | beta-D-fructose + ? | - |
? | |
| inulin + H2O | - |
Bifidobacterium longum subsp. infantis ATCC 15697 | beta-D-fructose + ? | - |
? | |
| raffinose + H2O | - |
Corynebacterium glutamicum | beta-D-fructose + melibiose | - |
? | |
| raffinose + H2O | - |
Corynebacterium glutamicum NRRL B-2312 | beta-D-fructose + melibiose | - |
? | |
| sucrose + H2O | - |
Bacillus cereus | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Arthrobacter globiformis | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Arthrobacter sp. | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Levilactobacillus brevis | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Paenibacillus macerans | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Thermotoga neapolitana | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Zymomonas mobilis | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Bifidobacterium breve | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Limosilactobacillus reuteri | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Bifidobacterium longum subsp. infantis | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Streptomyces sp. ALKC 8 | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | 10% of the activity as compared to raffinose | Corynebacterium glutamicum | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Limosilactobacillus reuteri CRL 1100 | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Arthrobacter globiformis IFO 3062 | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | 10% of the activity as compared to raffinose | Corynebacterium glutamicum NRRL B-2312 | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Bifidobacterium breve UCC2003 | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Thermotoga neapolitana DSM 4359T | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Bifidobacterium longum subsp. infantis ATCC 15697 | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Zymomonas mobilis CDBB-B 603 | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Bacillus cereus TA-11 | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Arthrobacter sp. 10137 | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Levilactobacillus brevis Mm-6 | beta-D-fructose + alpha-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Arthrobacter globiformis |
| 37 | - |
- |
Bifidobacterium breve |
| 37 | - |
- |
Limosilactobacillus reuteri |
| 37 | - |
- |
Bifidobacterium longum subsp. infantis |
| 40 | - |
- |
Corynebacterium glutamicum |
| 50 | - |
- |
Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermotoga neapolitana |
| 6 | - |
- |
Bifidobacterium breve |
| 6 | - |
- |
Limosilactobacillus reuteri |
| 6 | - |
- |
Bifidobacterium longum subsp. infantis |
| 6.8 | - |
- |
Corynebacterium glutamicum |
| 6.8 | - |
- |
Arthrobacter globiformis |
| 7 | - |
- |
Bacillus cereus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bifidobacterium longum subsp. infantis | - |
- |
4.3 |
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