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Literature summary for 3.2.1.26 extracted from
- Expression and characterization in E. coli of a neutral invertase from a metagenomic library (2010), World J. Microbiol. Biotechnol., 26, 419-428.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | uncultured bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sucrose | substrate inhibition, but retains 50% activity up to 1 M sucrose | uncultured bacterium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.46 | - |
sucrose | pH 7.0, 50°C | uncultured bacterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54870 | - |
x * 55000, SDS-PAGE, x * 54870, MALDI-MS | uncultured bacterium |
| 55000 | - |
x * 55000, SDS-PAGE, x * 54870, MALDI-MS | uncultured bacterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | C0LIF8 | uncultured bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestose + H2O | - |
uncultured bacterium | D-fructose + ? | - |
? | |
| additional information | enzyme does not show transfructosylating activity. No substrate: inulin | uncultured bacterium | ? | - |
? | |
| nystose + H2O | - |
uncultured bacterium | D-fructose + ? | - |
? | |
| raffinose + H2O | - |
uncultured bacterium | D-fructose + melibiose | - |
? | |
| sucrose + H2O | highest activity at 20 mM | uncultured bacterium | alpha-D-glucose + D-fructose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE, x * 54870, MALDI-MS | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Uninv | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
uncultured bacterium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 673 | - |
sucrose | pH 7.0, 50°C | uncultured bacterium | |
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