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Literature summary for 3.2.1.26 extracted from
- Inhibitors of plant invertases do not affect the structurally related enzymes of fructan metabolism (2009), New Phytol., 181, 601-612.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Arabidopsis thaliana cell wall/vacuolar inhibitor of fructosidase 2 | 122% activation of vacuolar invertase | Cichorium intybus | |
| Beta vulgaris cell wall/vacuolar inhibitor of fructosidase | 135% activation of vacuolar inveraase | Cichorium intybus | |
| Nicotiana tabacum vacuolar inhibitor of fructosidase | 154% activation of vacuolar invertase | Cichorium intybus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 48 h in Nicotiana benthamiana leaves infiltrated by Agrobacterium tumefaciens C58C1 with entry vector pDONR(TM)Zeo and destination vector pMDC32 | Cichorium intybus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Arabidopsis thaliana cell wall/vacuolar inhibitor of fructosidase 1 | cell-wall invertase: 69% activity of control | Cichorium intybus | |
| Arabidopsis thaliana cell wall/vacuolar inhibitor of fructosidase 2 | cell-wall invertase: 67% activity of control | Cichorium intybus | |
| Beta vulgaris cell wall/vacuolar inhibitor of fructosidase | cell-wall invertase: 57% activity of control | Cichorium intybus | |
| additional information | vacuolar invertase: no inhibition of Arabidopsis thaliana cell wall/vacuolar fructosidase 1 | Cichorium intybus | |
| Nicotiana tabacum cell wall inhibitor of fructosidase | cell-wall invertase: 28% activity of control; vacuolar invertase: 59% activity of control | Cichorium intybus | |
| Nicotiana tabacum vacuolar inhibitor of fructosidase | cell-wall invertase: 52% activity of control | Cichorium intybus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Cichorium intybus | 5618 | - |
| vacuole | - |
Cichorium intybus | 5773 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cichorium intybus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| leaf material frozen, ground, soluble proteins extracted, precipitated, dialyzed, purified by Con A chromatography, pH 6.8, to separate inhibitors from glycosylated invertase, invertase proteins bound to the Con A column are eluted with buffer containing 15% methyl-alpha-D-glucopyranoside | Cichorium intybus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.013 | - |
VI | Cichorium intybus |
| 0.143 | - |
CWI | Cichorium intybus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | 5 mM sucrose, 50 mM Na-acetate, pH 5.2, and 0.02% Na-azide, 30°C, 60 min | Cichorium intybus | D-glucose + D-fructose | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid invertase | - |
Cichorium intybus |
| cell-wall invertase | CWI | Cichorium intybus |
| Vacuolar invertase | VI | Cichorium intybus |
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Release 2023.1 (January 2023)
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