Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | slight activity increase | Uromyces viciae-fabae |
Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae and Pichia pastoris | Uromyces viciae-fabae |
General Stability | Organism |
---|---|
addition of up to 200 mM NaCl or 50 mM sucrose does not significantly alter INV1p stability | Uromyces viciae-fabae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | virtually abolishes invertase activity | Uromyces viciae-fabae | |
additional information | Mg2+ does not affect the activity | Uromyces viciae-fabae | |
Na+ | negatively effected by 0.8 M Na+ | Uromyces viciae-fabae | |
Zn2+ | slightly inhibitory | Uromyces viciae-fabae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27.1 | - |
sucrose | in 100 mM Na-acetate, pH 4.6, and 100 mM sucrose, at 35°C | Uromyces viciae-fabae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84300 | - |
estimated from amino acid sequence | Uromyces viciae-fabae |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | addition of up to 20% glycerol does not significantly alter INV1p stability | Uromyces viciae-fabae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Uromyces viciae-fabae | Q2PCS4 | precursor | - |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography | Uromyces viciae-fabae |
Storage Stability | Organism |
---|---|
-20°C, 4°C, or 20°C, in culture filtrate, several days, remains stable | Uromyces viciae-fabae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | sucrose is the only substrate | Uromyces viciae-fabae | D-glucose + D-fructose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-D-fructofuranoside fructohydrolase | - |
Uromyces viciae-fabae |
INV1p | - |
Uromyces viciae-fabae |
invertase | - |
Uromyces viciae-fabae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
slightly below 40°C | Uromyces viciae-fabae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
temperatures above 20°C result in a rapid loss of enzyme activity | Uromyces viciae-fabae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
- |
Uromyces viciae-fabae |