Cloned (Comment) | Organism |
---|---|
isozymes SAI and CWI, DNA and amino acid sequence determinations and analysis | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, isozymes SAI and CWI, isozyme Sai has a lower KM for sucrose and a higher KM for raffinose compared to isozyme CWI | Malus domestica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | cell wall-bound ivertase isozyme, CWI | Malus domestica | 5618 | - |
soluble | soluble acid invertase isozyme, SAI | Malus domestica | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, isozymes SAI and CWI, SDS-PAGE | Malus domestica |
220000 | - |
isozymes SAI and CWI | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
raffinose + H2O | Malus domestica | - |
D-fructose + melibiose | - |
ir | |
sucrose + H2O | Malus domestica | - |
D-glucose + D-fructose | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
two isozymes from cell wall and soluble fraction | - |
Purification (Comment) | Organism |
---|---|
native isozymes soluble acid invertase SAI and cell wall-bound invertase CWI from fruits to homogeneity | Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Malus domestica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity of isozymes SAI and CWI, no activity with stachyose of isozymes SAI and CWI | Malus domestica | ? | - |
? | |
raffinose + H2O | - |
Malus domestica | D-fructose + melibiose | - |
ir | |
sucrose + H2O | - |
Malus domestica | D-glucose + D-fructose | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, isozymes SAI and CWI, SDS-PAGE | Malus domestica |
Synonyms | Comment | Organism |
---|---|---|
cell wall-bound ivertase | - |
Malus domestica |
CWI | - |
Malus domestica |
invertase | - |
Malus domestica |
SAI | - |
Malus domestica |
soluble acid invertase | - |
Malus domestica |