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Literature summary for 3.2.1.26 extracted from

  • Win, T.T.; Isono, N.; Kusnadi, Y.; Watanabe, K.; Obae, K.; Ito, H.; Matsui, H.
    Enzymatic synthesis of two novel non-reducing oligosaccharides using transfructosylation activity with beta-fructofuranosidase from Arthrobacter globiformis (2004), Biotechnol. Lett., 26, 499-503.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
sucrose pH 7.0, 37°C Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, native enzyme, SDS-PAGE Arthrobacter globiformis

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-
Arthrobacter globiformis IFO 3062
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 37fold by ammonium sulfate fractionation, dialysis, and two steps of ion exchange chromatography Arthrobacter globiformis

Reaction

Reaction Comment Organism Reaction ID
raffinose + H2O = beta-D-fructofuranose + 6-O alpha-D-galactopyranosyl-alpha-D-glucopyranose the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism Arthrobacter globiformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101
-
purified enzyme Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism, overview Arthrobacter globiformis ?
-
?
additional information the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism, overview Arthrobacter globiformis IFO 3062 ?
-
?
sucrose + H2O
-
Arthrobacter globiformis D-glucose + D-fructose
-
?
sucrose + H2O
-
Arthrobacter globiformis IFO 3062 D-glucose + D-fructose
-
?

Subunits

Subunits Comment Organism
? x * 60000, native enzyme, SDS-PAGE Arthrobacter globiformis

Synonyms

Synonyms Comment Organism
beta-FFase
-
Arthrobacter globiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Arthrobacter globiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
15 min, purified enzyme, over 90% remaining activity Arthrobacter globiformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
127
-
sucrose pH 7.0, 37°C Arthrobacter globiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Arthrobacter globiformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.5 purified enzyme, over 90% of activity after 20 h at 4°C Arthrobacter globiformis