KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
227 | - |
sucrose | pH 4.5, 35°C | Rhodotorula glutinis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 3.4fold stimulation | Rhodotorula glutinis | |
Co2+ | 5 mM, 1.7fold stimulation | Rhodotorula glutinis | |
Cu2+ | 5 mM, 2fold stimulation | Rhodotorula glutinis | |
Fe2+ | 5 mM, 2.4fold stimulation | Rhodotorula glutinis | |
Mg2+ | 5 mM, 3.5fold stimulation | Rhodotorula glutinis | |
Na+ | 5 mM, 2fold stimulation | Rhodotorula glutinis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, SDS-PAGE | Rhodotorula glutinis |
100000 | - |
gel filtration | Rhodotorula glutinis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula glutinis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 19% carbohydrate | Rhodotorula glutinis |
Purification (Comment) | Organism |
---|---|
- |
Rhodotorula glutinis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
- |
Rhodotorula glutinis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no transfructosylation reraction at 1 M sucrose | Rhodotorula glutinis | ? | - |
? | |
raffinose + H2O | - |
Rhodotorula glutinis | beta-D-fructose + melibiose | - |
? | |
stachyose + H2O | - |
Rhodotorula glutinis | ? | - |
? | |
sucrose + H2O | - |
Rhodotorula glutinis | beta-D-fructose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, SDS-PAGE | Rhodotorula glutinis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life is 30 min | Rhodotorula glutinis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Rhodotorula glutinis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | pH 4: about 70% of maximal activity, pH 5.0: about 60% of maximal activity | Rhodotorula glutinis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.6 | 5.5 | stable | Rhodotorula glutinis |
6 | - |
30 min, 10% loss of activity | Rhodotorula glutinis |
8 | - |
30 min, 93% loss of activity | Rhodotorula glutinis |