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Literature summary for 3.2.1.26 extracted from

  • Belcarz, A.; Ginalska, G.; Lobarzewski, J.
    Immobilized, thermostable S- and F-forms of the extracellular invertase from Candida utilis can hydrolyse sucrose up to 100°C (2002), Biotechnol. Lett., 24, 1993-1998.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular when grown on sucrose-containing medium Candida utilis sectretes two invertases: the S-form of 280000 Da and the F-form of 62000 Da Cyberlindnera jadinii
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Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cyberlindnera jadinii

Storage Stability

Storage Stability Organism
4°C, sodium acetate buffer, pH 4.7, about 35% loss of activity of enzyme form S, about 15% loss of activity of enzyme form F Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
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Cyberlindnera jadinii beta-D-fructose + alpha-D-glucose
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?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 70 no defined optimum, soluble F-form Cyberlindnera jadinii
80
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soluble and immobilized S-form, immobilized F-form Cyberlindnera jadinii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 30°C: about 75% of maximal activity of soluble S-form, about 40% of maximal activity of immobilized S-form, about 60% of maximal activity of immobilized F-form, 90°C: about 20% of maximal activity of immobilized S-form, about 15% of maximal activity of soluble S-form, about 50% of maximal activity of immobilized F-form, about 30% of maximal activity of soluble F-form Cyberlindnera jadinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
90 min, about 25% loss of activity of S-form, F-form is stable Cyberlindnera jadinii
90
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90 min, about 45% loss of activity of S-form, F-form is stable Cyberlindnera jadinii
100
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20 min, about 35% loss of activity of S-form, about 15% loss of activity of the f-form Cyberlindnera jadinii