Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | when grown on sucrose-containing medium Candida utilis sectretes two invertases: the S-form of 280000 Da and the F-form of 62000 Da | Cyberlindnera jadinii | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera jadinii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cyberlindnera jadinii |
Storage Stability | Organism |
---|---|
4°C, sodium acetate buffer, pH 4.7, about 35% loss of activity of enzyme form S, about 15% loss of activity of enzyme form F | Cyberlindnera jadinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Cyberlindnera jadinii | beta-D-fructose + alpha-D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | no defined optimum, soluble F-form | Cyberlindnera jadinii |
80 | - |
soluble and immobilized S-form, immobilized F-form | Cyberlindnera jadinii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | 30°C: about 75% of maximal activity of soluble S-form, about 40% of maximal activity of immobilized S-form, about 60% of maximal activity of immobilized F-form, 90°C: about 20% of maximal activity of immobilized S-form, about 15% of maximal activity of soluble S-form, about 50% of maximal activity of immobilized F-form, about 30% of maximal activity of soluble F-form | Cyberlindnera jadinii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
90 min, about 25% loss of activity of S-form, F-form is stable | Cyberlindnera jadinii |
90 | - |
90 min, about 45% loss of activity of S-form, F-form is stable | Cyberlindnera jadinii |
100 | - |
20 min, about 35% loss of activity of S-form, about 15% loss of activity of the f-form | Cyberlindnera jadinii |