Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.26 extracted from

  • Acosta, N.; Beldarrain, A.; Rodriguez, L.; Alonso, Y.
    Characterization of recombinant invertase expressed in methylotrophic yeasts (2000), Biotechnol. Appl. Biochem., 32, 179-187.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Hansenula polymorpha and Pichia pastoris Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
enzyme expressed in Pichia pastoris, gel filtration Saccharomyces cerevisiae
190000
-
enzyme expressed in Hansenula polymorpha, gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein commercial enzyme contains 50% carbohydrate, enzyme expressed in Hansenula polymorpha contains 35% and enzyme expressed in Pichia pastoris contains 25% carbohydrate Saccharomyces cerevisiae

Renatured (Commentary)

Renatured (Comment) Organism
reactivation upon heating at 80°C depends on protein concentration. Irreversible denaturation is observed for carbohydrate-depleted enzyme Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3
-
enzyme expressed in Hansenula polymorpha Saccharomyces cerevisiae
3.4
-
enzyme expressed in Pichia pastoris Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
Saccharomyces cerevisiae beta-D-fructose + alpha-D-glucose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
enzyme expressed in Pichia pastoris Saccharomyces cerevisiae
60
-
enzyme expressed in Hansenula polymorpha Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: about 50% of maximal activity, 70°C: about 55% of maximal activity, enzyme expressed in Pichia pastoris Saccharomyces cerevisiae
40 70 40°C: about 55% of maximal activity, 70°C: about 60% of maximal activity, enzyme expressed in Hansenula polymorpha Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
180 min, more than 90% of initial activity of enzyme expressed in Hansenula polymorpha and Pichia pastoris remains Saccharomyces cerevisiae
60
-
10 min, enzyme expressed in Hansenula polymorpha shows optimal stability towards thermal inactivation at pH 5.5. Enzyme expressed in Pichia pastoris shows optimal stability towards thermal inactivation at pH 6 Saccharomyces cerevisiae