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Literature summary for 3.2.1.26 extracted from

  • Rubio, M.C.; Maldonado, M.C.
    Purification and characterization of invertase from Aspergillus niger (1995), Curr. Microbiol., 31, 80-83.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Aspergillus niger
Cu2+
-
Aspergillus niger
Hg2+
-
Aspergillus niger
K+
-
Aspergillus niger
Mg2+
-
Aspergillus niger
Na+
-
Aspergillus niger
NO3-
-
Aspergillus niger
PCMB 6 mM, complete inhibition Aspergillus niger
phosphate
-
Aspergillus niger
SO42-
-
Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0625
-
sucrose
-
Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
2 * 47000, SDS-PAGE Aspergillus niger
95000
-
gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
96
-
-
Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raffinose + H2O
-
Aspergillus niger fructose + melibiose
-
?
stachyose + H2O
-
Aspergillus niger ?
-
?
sucrose + H2O
-
Aspergillus niger D-fructose + D-glucose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 75 about 35% of maximal activity at 40°C and at 75°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 7 pH 4.0: about 60% of maximal activity, pH 7.0: about 25% of maximal activity Aspergillus niger
5
-
-
Aspergillus niger