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Literature summary for 3.2.1.26 extracted from
- Characterization and localization of three soluble invertase forms from Lilium longiflorum flower buds (1994), Physiol. Plant., 92, 247-253.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1.7 mM AgNO3, complete inhibition | Lilium longiflorum |  |
| Cu2+ | 1.7 mM, partial inhibition; CuSO4 | Lilium longiflorum | |
| Hg2+ | 1.7 mM HgCl2, complete inhibition | Lilium longiflorum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
sucrose | invertase I | Lilium longiflorum | |
| 1.6 | - |
raffinose | invertase I | Lilium longiflorum | |
| 6.4 | - |
sucrose | invertase II | Lilium longiflorum | |
| 6.6 | - |
sucrose | invertase III | Lilium longiflorum | |
| 10.8 | - |
raffinose | invertase II | Lilium longiflorum | |
| 13 | - |
raffinose | invertase III | Lilium longiflorum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 750000 | - |
gel filtration | Lilium longiflorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lilium longiflorum | - |
Thunb. cv. Nellie White | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Lilium longiflorum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lilium longiflorum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| anther | invertase I is primarily localized in anthers, invertase II and III are present in much smaller amounts | Lilium longiflorum | - |
| flower bud | invertase I is primarily localized in anthers, invertase II and II are present at 5% of invertase I activity. Much higher levels of invertase II and III are found in the nonanther organs of the flower | Lilium longiflorum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 112.4 | - |
invertase I | Lilium longiflorum |
| 253.6 | - |
invertase II | Lilium longiflorum |
| 466.1 | - |
invertase III | Lilium longiflorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no sucrose:sucrose fructosyltransferase activity | Lilium longiflorum | ? | - |
? | |
| raffinose + H2O | invertase I, at 45% of the activity with sucrose. Invertase II and III, at 30% of the activity with sucrose | Lilium longiflorum | fructose + melibiose | - |
? | |
| stachyose + H2O | invertase I, at 45% of the activity with sucrose. Invertase II and III, at 30% of the activity with sucrose | Lilium longiflorum | ? | - |
? | |
| sucrose + H2O | - |
Lilium longiflorum | D-fructose + D-glucose | - |
? | |
| trehalose + H2O | slow hydrolysis | Lilium longiflorum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
invertase I | Lilium longiflorum |
| 45 | - |
invertase III | Lilium longiflorum |
| 50 | - |
invertase II | Lilium longiflorum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, invertase II retains 25% of its activity. Invertase III retains 40% of its activity, invertase I completely loses activity | Lilium longiflorum |
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