Literature summary for 3.2.1.26 extracted from

Hayashi, S.; Shimokawa, Y.; Tubouchi, M.; Takasaki, Y.; Imada, K.
Properties of the deglycosylated beta-fructofuranosidase P-2 from Aureobasidium sp. ATCC 20524 (1992), J. Ind. Microbiol., 10, 191-194.

No PubMed abstract available

Search for more literature for 3.2.1.26

General Stability

General Stability	Organism
the deglycosylated enzyme is more sensitive than the native enzyme to proteases such as pronase E and subtilisin. The deglycosylated enzyme is more sensitive at pH 4 to 7 and 40°C to 50°C	Aureobasidium sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.65	-	sucrose	native enzyme	Aureobasidium sp.
1.43	-	sucrose	deglycosylated enzyme	Aureobasidium sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
216000	-	deglycosylated enzyme, gel filtration	Aureobasidium sp.
346000	-	native enzyme, gel filtration	Aureobasidium sp.

Organism

Organism	UniProt	Comment	Textmining
Aureobasidium sp.	-	ATCC 20524	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the carbohydrate moiety contributes to the stability as well as its affinity for sucrose	Aureobasidium sp.
glycoprotein	the native enzyme contains 53% w/w carbohydrate, the deglycosylated enzyme contains 15% w/w carbohydrate	Aureobasidium sp.

Purification (Commentary)

Purification (Comment)	Organism
-	Aureobasidium sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + H2O	-	Aureobasidium sp.	D-fructose + D-glucose	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	the deglycosylated enzyme is more sensitive than the glycosylated enzyme at 40-50°C	Aureobasidium sp.
50	-	15 min, native enzyme retains 98% of activity, deglycosylated enzyme retains 7% of its original activity	Aureobasidium sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	5.5	native enzyme	Aureobasidium sp.
5.5	-	deglycosylated enzyme	Aureobasidium sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
additional information	-	the deglycosylated enzyme is more sensitive at pH 4-7	Aureobasidium sp.

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Release 2023.1 (January 2023)