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Literature summary for 3.2.1.26 extracted from
- Wheat invertases. Characterization of cell wall-bound and soluble forms (1985), Plant Physiol., 78, 241-245.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 0.004 mM, complete inhibition; AgNO3 | Triticum aestivum |  |
| Hg2+ | 0.002 mM, complete inhibition; HgCl2 | Triticum aestivum | |
| I2 | 0.004 mM, complete inhibition | Triticum aestivum | |
| PCMB | - |
Triticum aestivum | |
| pyridoxal | 1 mM, 15% inhibition | Triticum aestivum | |
| pyridoxamine | 10 mM, 85% inhibition | Triticum aestivum | |
| pyridoxine | 2.5 mM, 24% inhibition | Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
sucrose | cell-wall bound enzyme | Triticum aestivum | |
| 3.5 | - |
sucrose | soluble enzyme | Triticum aestivum | |
| 19.6 | - |
raffinose | soluble enzyme | Triticum aestivum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | bound to | Triticum aestivum | 5618 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE | Triticum aestivum |
| 158000 | - |
gel filtration | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| soluble enzyme | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| coleoptile | a soluble and a cell wall-bound enzyme | Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1305 | - |
- |
Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raffinose + H2O | soluble enzyme | Triticum aestivum | fructose + melibiose | - |
? | |
| sucrose + H2O | - |
Triticum aestivum | D-fructose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Triticum aestivum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
4 min, soluble enzyme retains 60% of its activity, cell-wall bound enzyme retains only 20% of its activity | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
soluble enzyme | Triticum aestivum |
| 5.5 | - |
cell-wall bound enzyme | Triticum aestivum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | pH 4.0: about 70% of maximal activity, pH 5.0: about 55% of maximal activity, cell-wall bound enzyme | Triticum aestivum |
| 4.5 | 6 | about 70% of maximal activity at pH 4.5 and at pH 6.0 | Triticum aestivum |
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