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Literature summary for 3.2.1.26 extracted from

  • Nakagawa, H.; Kawasaki, Y.; Ogura, N.; Takehana, H.
    Purification and some properties of two types of beta-fructofuranosidase from tomato fruit (1971), Agric. Biol. Chem., 36, 18-26.
No PubMed abstract available
Search for more literature for 3.2.1.26

Inhibitors

Inhibitors Comment Organism Structure
Ag+ AgNO3 Solanum lycopersicum
Cu2+ CuSO4 Solanum lycopersicum
PCMB
-
 Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.17
-
 sucrose beta-FFasse-E, pH 5.1 Solanum lycopersicum
3.45
-
 sucrose beta-FFase E, pH 4.5 Solanum lycopersicum
3.7
-
 sucrose beta-FFase L, pH 5.1 Solanum lycopersicum
4.76
-
 sucrose beta-FFase L, pH 4.5 Solanum lycopersicum
5.89
-
 sucrose beta-FFase L, pH 3.6 Solanum lycopersicum
7.69
-
 sucrose beta-FFase E, pH 3.6 Solanum lycopersicum
10.6
-
 raffinose beta-FFase L, pH 5.1 Solanum lycopersicum
20.4
-
 raffinose beta-FFase E, pH 5.1 Solanum lycopersicum
21.7
-
 raffinose beta-FFase L, pH 3.6 Solanum lycopersicum
125
-
 raffinose beta-FFase E, pH 3.6 Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
K4Fe(CN)6 0.1 M, activates Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme form E and L Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raffinose + H2O
-
 Solanum lycopersicum fructose + melibiose
-
 ?
sucrose + H2O
-
 Solanum lycopersicum D-fructose + D-glucose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 5 min, complete inactivation Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 hydrolysis of sucrose Solanum lycopersicum
5.1
-
 hydrolysis of raffinose Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
2.7 5.7 pH 2.7: about 55% of maximal activity, pH 5.7: about 70% of maximal activity, hydrolysis of sucrose Solanum lycopersicum
3.7 5.5 about 75% of maximal activity, pH 5.5: about 50% of maximal activity Solanum lycopersicum