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Literature summary for 3.2.1.26 extracted from

  • Goldstein, A.; Lampen, J.O.
    beta-D-Fructofuranoside fructohydrolase from yeast (1975), Methods Enzymol., 42C, 504-511.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin stimulates intracellular enzyme Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
2-Amino-2-hydroxymethyl propane-1,3-diol extracellular enzyme Saccharomyces cerevisiae
ammonium sulfate intracellular enzyme Saccharomyces cerevisiae
aniline extracellular enzyme Saccharomyces cerevisiae
I2 extracellular enzyme Saccharomyces cerevisiae
NaCl 0.1 M, partial, intracellular enzyme Saccharomyces cerevisiae
Sodium sulfate 0.1 M, partial, intracellular enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
25 26 sucrose extracellular enzyme Saccharomyces cerevisiae
150
-
raffinose exteracellular enzyme Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
extracellular invertase and intracellular invertase
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein extracellular enzyme contains up to 50% carbohydrate Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
intracellular and extracellular invertase Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4700
-
intracellular enzyme Saccharomyces cerevisiae
4800
-
extracellular enzyme Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
below 0°C, lyophilized enzyme, stable for at least 1 year, extracellular enzyme Saccharomyces cerevisiae
frozen in solution, pH 4.5-5.0, stable for a few months, extracellular enzyme Saccharomyces cerevisiae
frozen, pH 7.0-7.5, stable for a few months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl-beta-D-fructofuranoside + H2O
-
Saccharomyces cerevisiae ?
-
?
additional information at high substrate concentrations, 1 M, transferase activity, transferring the beta-D-fructofuranosyl residue to primary alcohols such as methanol, ethanol and n-propanol, extracellular enzyme Saccharomyces cerevisiae ?
-
?
raffinose + H2O
-
Saccharomyces cerevisiae fructose + melibiose
-
?
sucrose + H2O
-
Saccharomyces cerevisiae D-fructose + D-glucose beta-D-fructofuranose ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 3-7, 2 h, extracellular enzyme, stable Saccharomyces cerevisiae
56
-
15 min stable at pH 4.9, very unstable above pH 6, extracellular enzyme. Intracellular enzyme is unstable above pH 6, stable at pH 4.9 for 10 min Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 5 extracellular and intracellular enzyme Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 30°C, 2 h, extracellular enzyme, stable Saccharomyces cerevisiae
6.5 9 30°C, 2 h, intracellular enzyme, stable. In presence of bovine serum albumin stable down to pH 4.9 Saccharomyces cerevisiae