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Literature summary for 3.2.1.25 extracted from
- beta-Mannosidase from the mushroom Polyporus sulfureus (1976), J. Biol. Chem., 251, 4384-4388.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 2 mM, complete inhibition | Polyporus sulfureus |  |
| Cu2+ | - |
Polyporus sulfureus | |
| D-mannose | - |
Polyporus sulfureus | |
| Fe3+ | - |
Polyporus sulfureus | |
| Hg2+ | - |
Polyporus sulfureus | |
| PMSF | - |
Polyporus sulfureus | |
| Zn2+ | - |
Polyporus sulfureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
p-nitrophenyl-beta-D-mannoside | - |
Polyporus sulfureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
gel filtration | Polyporus sulfureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Polyporus sulfureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Polyporus sulfureus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit body | - |
Polyporus sulfureus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
- |
Polyporus sulfureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta1-4-mannobiose + H2O | - |
Polyporus sulfureus | ? | - |
? | |
| Man-(beta1-4)GlcNAc + H2O | - |
Polyporus sulfureus | mannose + GlcNAc | - |
? | |
| Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta-Asn + H2O | - |
Polyporus sulfureus | mannose + GlcNAcbeta(1-4)GlcNAcbeta-Asn | - |
? | |
| mannotriose + H2O | - |
Polyporus sulfureus | ? | - |
? | |
| additional information | the enzyme liberates mannose from core glycopeptide isolated from Taka-amylase A and alpha1-acid glycoprotein | Polyporus sulfureus | ? | - |
? | |
| p-nitrophenyl-beta-D-mannoside + H2O | - |
Polyporus sulfureus | p-nitrophenol + alpha-D-mannose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
hydrolysis of Manbeta1-4GlcNAcbeta1-4GlcNAcbeta-Asn | Polyporus sulfureus |
| 2.8 | - |
hydrolysis of p-nitrophenyl-beta-D-mannoside or beta-1,3-mannobiose | Polyporus sulfureus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.8 | 9 | 25°C, 1 h, stable | Polyporus sulfureus |
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