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Literature summary for 3.2.1.25 extracted from

  • Akino, T.; Nakamura, N.; Horikoshi, K.
    Characterization of beta-mannosidase of an alkalophilic Bacillus sp. (1988), Agric. Biol. Chem., 52, 1459-1464.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Bacillus sp. (in: Bacteria)
Cd2+
-
Bacillus sp. (in: Bacteria)
Cu2+
-
Bacillus sp. (in: Bacteria)
Hg2+
-
Bacillus sp. (in: Bacteria)
N-bromosuccinimide
-
Bacillus sp. (in: Bacteria)
n-Dodecylbenzene sulfonate
-
Bacillus sp. (in: Bacteria)
PCMB
-
Bacillus sp. (in: Bacteria)
SDS
-
Bacillus sp. (in: Bacteria)
Zn2+
-
Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
p-nitrophenyl-beta-D-mannopyranoside
-
Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
x * 94000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
AM-001
-
Bacillus sp. (in: Bacteria) AM-001
-
AM-001
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
-
Bacillus sp. (in: Bacteria) D-mannopyranose
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
-
Bacillus sp. (in: Bacteria) AM-001 D-mannopyranose
-
?
mannopentaose + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
mannopentaose + H2O
-
Bacillus sp. (in: Bacteria) AM-001 ?
-
?
mannotetraose + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
mannotetraose + H2O
-
Bacillus sp. (in: Bacteria) AM-001 ?
-
?
mannotriose + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
mannotriose + H2O
-
Bacillus sp. (in: Bacteria) AM-001 ?
-
?
p-nitrophenyl-beta-D-mannoside + H2O p-nitrophenyl-beta-D-mannopyranoside Bacillus sp. (in: Bacteria) p-nitrophenol + alpha-D-mannose
-
?
p-nitrophenyl-beta-D-mannoside + H2O p-nitrophenyl-beta-D-mannopyranoside Bacillus sp. (in: Bacteria) AM-001 p-nitrophenol + alpha-D-mannose
-
?

Subunits

Subunits Comment Organism
? x * 94000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 35% of maximal activity, 60°C: about 55% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8 40°C, 30 min, stable. Unstable below pH 6.5 and above pH 8.0 Bacillus sp. (in: Bacteria)