Any feedback?
Literature summary for 3.2.1.25 extracted from
- beta-D-Mannosidase (1988), Methods Enzymol., 160, 614-619.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme together with alpha-galactosidase and endo-beta-D-mannanase, can be used to characterize the structures of more complex oligosacharides | Helix pomatia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Helix pomatia |  |
| Hg2+ | - |
Helix pomatia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.91 | - |
4-methylumbelliferyl beta-D-mannopyranoside | - |
Helix pomatia | |
| 1.43 | - |
p-nitrophenyl-beta-D-mannopyranoside | - |
Helix pomatia | |
| 2.33 | - |
o-nitrophenyl-beta-D-mannopyranoside | - |
Helix pomatia | |
| 3.22 | - |
Naphthyl-beta-D-mannopyranoside | - |
Helix pomatia | |
| 12.5 | - |
beta-D-mannotetraitol | - |
Helix pomatia | |
| 12.5 | - |
Beta-D-mannohexaitol | - |
Helix pomatia | |
| 12.5 | - |
beta-D-mannopentaitol | - |
Helix pomatia | |
| 12.5 | - |
beta-D-mannotriitol | - |
Helix pomatia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
- |
Helix pomatia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helix pomatia | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 3.1% carbohydrate | Helix pomatia |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Helix pomatia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Helix pomatia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-mannoside + H2O | - |
Helix pomatia | 4-methylumbelliferone + beta-D-mannose | - |
? | |
| beta-D-mannohexaitol + H2O | - |
Helix pomatia | ? | - |
? | |
| beta-D-mannopentaitol + H2O | - |
Helix pomatia | ? | - |
? | |
| beta-D-mannotetraitol + H2O | - |
Helix pomatia | ? | - |
? | |
| beta-D-mannotriitol + H2O | - |
Helix pomatia | ? | - |
? | |
| beta-mannotetraitol + H2O | - |
Helix pomatia | ? | - |
? | |
| naphthyl-beta-D-mannopyranoside + H2O | - |
Helix pomatia | 2-naphthol + beta-D-mannose | - |
? | |
| o-nitrophenyl-beta-D-mannopyranoside + H2O | - |
Helix pomatia | o-nitrophenol + alpha-D-mannose | - |
? | |
| p-nitrophenyl-beta-D-mannoside + H2O | p-nitrophenyl-beta-D-mannopyranoside | Helix pomatia | p-nitrophenol + alpha-D-mannose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 94000, SDS-PAGE | Helix pomatia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable to extended incubation at pH 4.5 | Helix pomatia |
| 50 | - |
rapid loss of activity above | Helix pomatia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Helix pomatia |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
stable to extended incubation at 45°C | Helix pomatia |
| 7 | - |
unstable above pH 7 | Helix pomatia |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
