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Literature summary for 3.2.1.24 extracted from

  • Cobucci-Ponzano, B.; Conte, F.; Strazzulli, A.; Capasso, C.; Fiume, I.; Pocsfalvi, G.; Rossi, M.; Moracci, M.
    The molecular characterization of a novel GH38 alpha-mannosidase from the crenarchaeon Sulfolobus solfataricus revealed its ability in de-mannosylating glycoproteins (2010), Biochimie, 92, 1895-1907.
    View publication on PubMed
Search for more literature for 3.2.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a fusion of the glutathione S-transferase of Schistosoma japonicum in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
D338G inactive mutant enzyme. In presence of the external nucleophil (1 M) sodium formate the activity is partially rescued Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
additional information activity is insensitive to 1-deoxymannojirimycin at concentration up to 2 mM. No change in the enzymatic activity is observed with 0.001-1 mM EDTA Saccharolobus solfataricus
swainsonine
-
 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 4-nitrophenyl alpha-D-mannoside pH 6.5, 65°C, recombinant enzyme Saccharolobus solfataricus
0.45
-
 4-nitrophenyl alpha-D-mannoside pH 6.5, 65°C, native enzyme Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 0.01 and 0.1 mM, activates recombinant enzyme Saccharolobus solfataricus
Co2+ 0.01 and 0.1 mM, activates recombinant enzyme Saccharolobus solfataricus
Mg2+ 0.1 mM, activates recombinant enzyme Saccharolobus solfataricus
Mn2+ 1 mM, activates recombinant enzyme Saccharolobus solfataricus
additional information no change in the enzymatic activity is observed with Cu2+ or Ni2+ at 0.001-1 mM Saccharolobus solfataricus
Zn2+ the enzyme binds Zn2+ in vivo, it contains 2.9 Zn2+ per trimeric enzyme, corresponding to a single atom per subunit. 0.1 mM Zn2+ showes 2fold activation of recombinant enzyme Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 3 * 110000, SDS-PAGE Saccharolobus solfataricus
363000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharolobus solfataricus the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein ?
-
 ?
additional information Saccharolobus solfataricus P2 the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97UK5
-
-
Saccharolobus solfataricus P2 Q97UK5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.4
-
 substrate: 4-nitrophenyl alpha-D-mannoside, pH 6.5, 65°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-mannoside + H2O no hydrolysis observed on 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl alpha-D-xyloside, 4-nitrophenyl alpha-L-arabinoside, 4-nitrophenyl alpha-L-rhamnoside, 4-nitrophenyl alpha-L-fucoside, and 4-nitrophenyl beta-L-fucoside Saccharolobus solfataricus 4-nitrophenol + alpha-D-mannose
-
 ?
4-nitrophenyl alpha-D-mannoside + H2O no hydrolysis observed on 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl alpha-D-xyloside, 4-nitrophenyl alpha-L-arabinoside, 4-nitrophenyl alpha-L-rhamnoside, 4-nitrophenyl alpha-L-fucoside, and 4-nitrophenyl beta-L-fucoside Saccharolobus solfataricus P2 4-nitrophenol + alpha-D-mannose
-
 ?
alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O
-
 Saccharolobus solfataricus alpha-D-mannose + ?
-
 ?
alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O
-
 Saccharolobus solfataricus P2 alpha-D-mannose + ?
-
 ?
alpha-D-Man-(1->2)-D-Man + H2O
-
 Saccharolobus solfataricus 2 alpha-D-mannose
-
 ?
alpha-D-Man-(1->2)-D-Man + H2O
-
 Saccharolobus solfataricus P2 2 alpha-D-mannose
-
 ?
alpha-D-Man-(1->3)-D-Man + H2O
-
 Saccharolobus solfataricus 2 alpha-D-mannose
-
 ?
alpha-D-Man-(1->3)-D-Man + H2O
-
 Saccharolobus solfataricus P2 2 alpha-D-mannose
-
 ?
alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O
-
 Saccharolobus solfataricus alpha-D-mannose + ?
-
 ?
alpha-D-Man-(1->6)-D-Man + H2O
-
 Saccharolobus solfataricus 2 alpha-D-mannose
-
 ?
additional information the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein Saccharolobus solfataricus ?
-
 ?
additional information the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein Saccharolobus solfataricus P2 ?
-
 ?
RNase-B-Man(n)-glycoform + H2O
-
 Saccharolobus solfataricus RNase-B-Man(n-1)-glycoform + alpha-D-mannose
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 110000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
GH38 alpha-mannosidase
-
 Saccharolobus solfataricus
Ssa-man
-
 Saccharolobus solfataricus
SSO3006 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Saccharolobus solfataricus
85
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life: 1.5 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 4-nitrophenyl alpha-D-mannoside pH 6.5, 65°C, recombinant enzyme Saccharolobus solfataricus
158
-
 4-nitrophenyl alpha-D-mannoside pH 6.5, 65°C, native enzyme Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Saccharolobus solfataricus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.2
-
 pH 6.5, 65°C Saccharolobus solfataricus swainsonine

General Information

General Information Comment Organism
physiological function the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
54
-
 4-nitrophenyl alpha-D-mannoside pH 6.5, 65°C, recombinant enzyme Saccharolobus solfataricus
351
-
 4-nitrophenyl alpha-D-mannoside pH 6.5, 65°C, native enzyme Saccharolobus solfataricus