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Literature summary for 3.2.1.24 extracted from

  • Angelov, A.; Putyrski, M.; Liebl, W.
    Molecular and biochemical characterization of alpha-glucosidase and alpha-mannosidase and their clustered genes from the thermoacidophilic archaeon Picrophilus torridus (2006), J. Bacteriol., 188, 7123-7131.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Picrophilus torridus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong inhibitor at concentrations of 1 mM or 10 mM Picrophilus torridus
Cu2+ strong inhibitor at concentrations of 1 mM or 10 mM Picrophilus torridus
EDTA supplied in equimolar amounts or in excess, neutralizes also effect of resistance of ManA to heat inactivation Picrophilus torridus
Fe3+ strong inhibitor at concentrations of 1 mM or 10 mM Picrophilus torridus
additional information 1-deoxymannojirimycin has no effect on ManA at concentrations up to 500 microM Picrophilus torridus
Ni2+ strong inhibitor at concentrations of 1 mM or 10 mM Picrophilus torridus
swainsonine shows competitive inhibition when p-nitrophenyl-Man is used as a substrate Picrophilus torridus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ potent activator at concentration of 1 mM, 12.5fold activation compared to the apoenzyme Picrophilus torridus
Co2+ 8.3fold activation at a concentration of 10 mM, resistance of ManA to heat inactivation is increased by presence of Co2+ Picrophilus torridus
Fe2+ 4.5fold activation at a concentration of 10 mM Picrophilus torridus
Mn2+ 3.8fold activation at a concentration of 10 mM Picrophilus torridus
additional information Zn2+, Ca2+, Mg2+, Ba2+, K+, and Li+ had no significant effect on ManA activity at a concentration of 1 mM or 10 mM Picrophilus torridus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
111500
-
2 * 111500, gel filtration Picrophilus torridus
120000
-
small fraction of ManA activity, reflecting single subunits Picrophilus torridus
220000
-
gel filtration Picrophilus torridus

Organism

Organism UniProt Comment Textmining
Picrophilus torridus Q6L2X5 extremophilic organism belonging to the euryarchaeal phylum
-

Purification (Commentary)

Purification (Comment) Organism
SDS-PAGE, anion-exchange, size exclusion chromatography Picrophilus torridus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.6
-
ManA is equally active with mannobiose substrates, released mannose is quantified, resulting in a specific activity of approximately 6.6 U/mg Picrophilus torridus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-alpha-D-mannobiose + H2O
-
Picrophilus torridus alpha-D-mannose
-
?
1,3-alpha-D-mannobiose + H2O
-
Picrophilus torridus alpha-D-mannose
-
?
1,6-alpha-D-mannobiose + H2O
-
Picrophilus torridus alpha-D-mannose
-
?
alpha3,alpha6-D-mannopentaose + H2O
-
Picrophilus torridus alpha-D-mannose
-
?
methyl-O-1,4-alpha-D-mannobiose + H2O
-
Picrophilus torridus alpha-D-mannose
-
?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
Picrophilus torridus 4-nitrophenol + alpha-D-mannose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 111500, gel filtration Picrophilus torridus

Synonyms

Synonyms Comment Organism
alpha-mannosidase
-
Picrophilus torridus
ManA
-
Picrophilus torridus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
maximum activity of ManA Picrophilus torridus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
resistance of ManA to heat inactivation (at 80°C) Picrophilus torridus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
maximum activity of ManA Picrophilus torridus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00122
-
swainsonine
-
Picrophilus torridus