Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Picrophilus torridus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibitor at concentrations of 1 mM or 10 mM | Picrophilus torridus | |
Cu2+ | strong inhibitor at concentrations of 1 mM or 10 mM | Picrophilus torridus | |
EDTA | supplied in equimolar amounts or in excess, neutralizes also effect of resistance of ManA to heat inactivation | Picrophilus torridus | |
Fe3+ | strong inhibitor at concentrations of 1 mM or 10 mM | Picrophilus torridus | |
additional information | 1-deoxymannojirimycin has no effect on ManA at concentrations up to 500 microM | Picrophilus torridus | |
Ni2+ | strong inhibitor at concentrations of 1 mM or 10 mM | Picrophilus torridus | |
swainsonine | shows competitive inhibition when p-nitrophenyl-Man is used as a substrate | Picrophilus torridus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | potent activator at concentration of 1 mM, 12.5fold activation compared to the apoenzyme | Picrophilus torridus | |
Co2+ | 8.3fold activation at a concentration of 10 mM, resistance of ManA to heat inactivation is increased by presence of Co2+ | Picrophilus torridus | |
Fe2+ | 4.5fold activation at a concentration of 10 mM | Picrophilus torridus | |
Mn2+ | 3.8fold activation at a concentration of 10 mM | Picrophilus torridus | |
additional information | Zn2+, Ca2+, Mg2+, Ba2+, K+, and Li+ had no significant effect on ManA activity at a concentration of 1 mM or 10 mM | Picrophilus torridus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
111500 | - |
2 * 111500, gel filtration | Picrophilus torridus |
120000 | - |
small fraction of ManA activity, reflecting single subunits | Picrophilus torridus |
220000 | - |
gel filtration | Picrophilus torridus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | Q6L2X5 | extremophilic organism belonging to the euryarchaeal phylum | - |
Purification (Comment) | Organism |
---|---|
SDS-PAGE, anion-exchange, size exclusion chromatography | Picrophilus torridus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.6 | - |
ManA is equally active with mannobiose substrates, released mannose is quantified, resulting in a specific activity of approximately 6.6 U/mg | Picrophilus torridus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-alpha-D-mannobiose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
1,3-alpha-D-mannobiose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
1,6-alpha-D-mannobiose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
alpha3,alpha6-D-mannopentaose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
methyl-O-1,4-alpha-D-mannobiose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Picrophilus torridus | 4-nitrophenol + alpha-D-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 111500, gel filtration | Picrophilus torridus |
Synonyms | Comment | Organism |
---|---|---|
alpha-mannosidase | - |
Picrophilus torridus |
ManA | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
maximum activity of ManA | Picrophilus torridus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
resistance of ManA to heat inactivation (at 80°C) | Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
maximum activity of ManA | Picrophilus torridus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00122 | - |
swainsonine | - |
Picrophilus torridus |