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Literature summary for 3.2.1.24 extracted from

  • Muramatsu, T.; Egami, F.
    alpha-Mannosidase and beta-mannosidase from the liver of Turbo cortunus: Purification, properties and application in carbohydrate research (1967), J. Biochem., 62, 700-709.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NaCl 50% activation up to 1.5 M Turbo cornutus

Inhibitors

Inhibitors Comment Organism Structure
cysteine 31% inhibition at 0.02 mM Turbo cornutus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
p-nitrophenyl-alpha-D-mannopyranoside
-
Turbo cornutus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ slight activation at 0.02 mM Turbo cornutus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycopeptide + H2O Turbo cornutus from ovalbumin alpha-D-mannopyranose + ?
-
?
mannosyl rhamnose + H2O Turbo cornutus part of the repeating oligosaccharide unit of the O-antigenic lipopolysaccharide in Salmonella typhimurium alpha-D-mannopyranose + rhamnose
-
?
TAKA-amylase A + H2O Turbo cornutus
-
alpha-D-mannopyranose + ?
-
?

Organism

Organism UniProt Comment Textmining
Turbo cornutus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, chromatography steps Turbo cornutus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Turbo cornutus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycopeptide + H2O from ovalbumin Turbo cornutus alpha-D-mannopyranose + ?
-
?
mannosyl rhamnose + H2O part of the repeating oligosaccharide unit of the O-antigenic lipopolysaccharide in Salmonella typhimurium Turbo cornutus alpha-D-mannopyranose + rhamnose
-
?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
Turbo cornutus p-nitrophenol + alpha-D-mannopyranose
-
?
TAKA-amylase A + H2O
-
Turbo cornutus alpha-D-mannopyranose + ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20
-
no loss of activity within 3 months Turbo cornutus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Turbo cornutus