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Literature summary for 3.2.1.24 extracted from

  • Matta, K.L.; Bahl, O.P.
    Glycosidases of Aspergillus niger. IV. Purification and characterization of alpha-mannosidase (1972), J. Biol. Chem., 247, 1780-1787.
    View publication on PubMed
Search for more literature for 3.2.1.24

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Aspergillus niger
Ba2+ inhibition at 1 mM Aspergillus niger
Ca2+ inhibition at 0.1 mM Aspergillus niger
Cd2+ inhibition at 0.1 mM Aspergillus niger
Cu2+ inhibition at 0.1 mM Aspergillus niger
D-mannono-1,5-lactone complete inhibition at 1 mM Aspergillus niger
Fe3+ inhibition at 1 mM Aspergillus niger
Hg2+ strong inhibitor Aspergillus niger
Mg2+ inhibition at 1 mM Aspergillus niger
Mn2+ inhibition at 1 mM Aspergillus niger
Sr2+ inhibition at 1 mM Aspergillus niger
Zn2+ inhibition at 1 mM Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Aspergillus niger
8.2
-
 methyl-4-O-alpha-D-mannopyranosyl-D-mannopyranoside
-
 Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O Aspergillus niger very low activity 2 alpha-D-mannopyranose
-
 ?
3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O Aspergillus niger very low activity 2 alpha-D-mannopyranose
-
 ?
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O Aspergillus niger specific cleavage of alpha-1,4- or alpha-1,6-linkages 2 alpha-D-mannopyranose
-
 ?
6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O Aspergillus niger specific cleavage of alpha-1,4- or alpha-1,6-linkages alpha-D-mannopyranose
-
 ?
glycopeptide + H2O Aspergillus niger faster hydrolysis than for glycoprotein alpha-D-mannopyranose + ?
-
 ?
glycoprotein + H2O Aspergillus niger
-
 alpha-D-mannopyranose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation Rhozyme HP-150, crude enzyme preparation Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetamido-4-O-alpha-D-mannopyranosyl-2-deoxy-D-glucose + H2O
-
 Aspergillus niger alpha-D-mannopyranose + ?
-
 ?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O very low activity Aspergillus niger alpha-D-mannopyranose + D-mannopyranose
-
 ?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O very low activity Aspergillus niger 2 alpha-D-mannopyranose
-
 ?
3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O very low activity Aspergillus niger 2 alpha-D-mannopyranose
-
 ?
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O specific cleavage of alpha-1,4- or alpha-1,6-linkages Aspergillus niger 2 alpha-D-mannopyranose
-
 ?
6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O specific cleavage of alpha-1,4- or alpha-1,6-linkages Aspergillus niger alpha-D-mannopyranose
-
 ?
glycopeptide + H2O faster hydrolysis than for glycoprotein Aspergillus niger alpha-D-mannopyranose + ?
-
 ?
glycoprotein + H2O
-
 Aspergillus niger alpha-D-mannopyranose + ?
-
 ?
methyl-2-O-alpha-D-mannopyranosyl-D-mannopyranoside + H2O very low activity Aspergillus niger alpha-D-mannopyranose + ?
-
 ?
methyl-4-O-alpha-D-mannopyranosyl-D-mannopyranoside + H2O
-
 Aspergillus niger alpha-D-mannopyranose + ?
-
 ?
p-nitrophenyl-alpha-D-mannopyranoside + H2O very low activity Aspergillus niger p-nitrophenol + alpha-D-mannopyranose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 45 stable within Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 stable within Aspergillus niger