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Literature summary for 3.2.1.24 extracted from
- alpha-D-Mannosidase. Preparation and properties of free and insolubilized enzyme (1976), Biochim. Biophys. Acta, 429, 884-894.
General Stability
| General Stability | Organism |
|---|---|
| 25-30% of activity remains in 6 M urea | Canavalia ensiformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucuronic acid | slight inhibition at 10 mM | Canavalia ensiformis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
2 * 66000 + 2 * 44000, SDS-PAGE | Canavalia ensiformis |
| 66000 | - |
2 * 66000 + 2 * 44000, SDS-PAGE | Canavalia ensiformis |
| 200000 | - |
gel filtration, sucrose density gradient centrifugation | Canavalia ensiformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycopeptide + H2O | Canavalia ensiformis | from ovalbumin | alpha-D-mannopyranose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canavalia ensiformis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Canavalia ensiformis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Canavalia ensiformis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| 66% remaining activity after urea is removed from a Sepharose-immobilized enzyme | Canavalia ensiformis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Canavalia ensiformis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Canavalia ensiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycopeptide + H2O | from ovalbumin | Canavalia ensiformis | alpha-D-mannopyranose + ? | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Canavalia ensiformis | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 66000 + 2 * 44000, SDS-PAGE | Canavalia ensiformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
continual increase of activity up to | Canavalia ensiformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Canavalia ensiformis |
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Release 2023.1 (January 2023)
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