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Literature summary for 3.2.1.24 extracted from

  • Kaya, T.; Aikawa, M.; Matsumoto, T.
    Purification and properties of alpha-mannosidase from baker's yeast (1977), J. Biochem., 82, 1443-1449.
    View publication on PubMed
Search for more literature for 3.2.1.24

Activating Compound

Activating Compound Comment Organism Structure
Mannan from yeast, 80% activation at 10 mg/ml Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid slight inhibition at 0.1 mM Saccharomyces cerevisiae
methyl-alpha-D-mannopyranoside 37% inhibition at 0.1 mM Saccharomyces cerevisiae
NaN3 slight inhibition at 0.1 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Saccharomyces cerevisiae
0.34
-
 methyl-alpha-D-mannopyranoside
-
 Saccharomyces cerevisiae
0.42
-
 phenyl-alpha-D-mannopyranoside
-
 Saccharomyces cerevisiae
0.67
-
 Mannotetraose
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 28% stimulation at 0.1 mM Saccharomyces cerevisiae
Mg2+ 50% stimulation at 0.1 mM Saccharomyces cerevisiae
Zn2+ 90% stimulation at 0.1 mM Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
450000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mannan + H2O Saccharomyces cerevisiae very low activity alpha-D-mannose + ?
-
 ?
mannan + H2O Saccharomyces cerevisiae from yeast alpha-D-mannose + ?
-
 ?
mannotetraose + H2O Saccharomyces cerevisiae
-
 alpha-D-mannopyranose + mannotriose
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
5°C, 50% loss of activity after 3 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mannan + H2O very low activity Saccharomyces cerevisiae alpha-D-mannose + ?
-
 ?
mannan + H2O from yeast Saccharomyces cerevisiae alpha-D-mannose + ?
-
 ?
mannotetraose + H2O
-
 Saccharomyces cerevisiae alpha-D-mannopyranose + mannotriose
-
 ?
methyl-alpha-D-mannopyranoside + H2O
-
 Saccharomyces cerevisiae alpha-D-mannopyranose + methanol
-
 ?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Saccharomyces cerevisiae p-nitrophenol + alpha-D-mannopyranose
-
 ?
phenyl-alpha-D-mannopyranoside + H2O
-
 Saccharomyces cerevisiae alpha-D-mannopyranose + phenol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
 stable for 5 min Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.8
-
 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 unstable below Saccharomyces cerevisiae