Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 97% inhibition at 10 mM | Lupinus angustifolius | |
alpha-conglutin | 11% inhibition at saturation | Lupinus angustifolius | |
alpha-D-mannopyranose | 29% inhibition at 10 mM | Lupinus angustifolius | |
Cu2+ | 10% inhibition at 10 mM, alpha-mannosidase A | Lupinus angustifolius | |
EDTA | addition of Zn2+ restores inhibitory effect | Lupinus angustifolius | |
Hg2+ | 45% inhibition at 10 mM | Lupinus angustifolius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.92 | - |
p-nitrophenyl-alpha-D-mannopyranoside | alpha-mannosidase A | Lupinus angustifolius | |
2.7 | - |
p-nitrophenyl-alpha-D-mannopyranoside | alpha-mannosidase B | Lupinus angustifolius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
protein storage vacuole | - |
Lupinus angustifolius | 326 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | maximum activity at 1 mM | Lupinus angustifolius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
gel filtration, alpha-mannosidases A and B | Lupinus angustifolius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-conglutin + H2O | Lupinus angustifolius | lupin seed storage glycoprotein, not active to beta- or gamma-conglutin | alpha-D-mannopyranose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus angustifolius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Lupinus angustifolius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | alpha-mannosidases A and B | Lupinus angustifolius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.93 | - |
alpha-mannosidase B | Lupinus angustifolius |
15.94 | - |
alpha-mannosidase A | Lupinus angustifolius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-conglutin + H2O | lupin seed storage glycoprotein, not active to beta- or gamma-conglutin | Lupinus angustifolius | alpha-D-mannopyranose + ? | - |
? | |
methyl-alpha-D-mannnopyranose + H2O | - |
Lupinus angustifolius | alpha-D-mannopyranose + methanol | - |
? | |
p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Lupinus angustifolius | p-nitrophenol + alpha-D-mannopyranose | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
alpha-mannosidase A | Lupinus angustifolius |
4.6 | - |
alpha-mannosidase B | Lupinus angustifolius |