General Stability | Organism |
---|---|
stable in the presence of Co2+ at 37°C | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition at 1 mM | Rattus sp. | |
EDTA | strong inhibition at 1 mM | Rattus sp. | |
Fe2+ | slight inhibition at 1 mM | Rattus sp. | |
iodoacetamide | slight inhibition at 1 mM | Rattus sp. | |
p-chloromercuribenzenesulfonic acid | strong inhibition at 1 mM | Rattus sp. | |
swainsonine | 50% inhibition in the range of 0.3-0.6 mM | Rattus sp. | |
Zn2+ | strong inhibition at 1 mM | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
Man5GlcNAc | - |
Rattus sp. | |
0.11 | - |
Man9GlcNAc | - |
Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | from microsomal membranes | Rattus sp. | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 20% activation at 1 mM | Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
2 * 110000, reducing and non-reducing SDS-PAGE | Rattus sp. |
220000 | - |
gel filtration | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O | Rattus sp. | - |
6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc | end products | ? | |
Man6GlcNAc + H2O | Rattus sp. | ordered hydrolysis of alpha(1-2)-residues followed by hydrolysis of alpha(1-3)- and alpha(1-6)-linked residues | alpha-D-mannopyranose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.27 | - |
- |
Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O | - |
Rattus sp. | 6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc | end products | ? | |
Man6GlcNAc + H2O | ordered hydrolysis of alpha(1-2)-residues followed by hydrolysis of alpha(1-3)- and alpha(1-6)-linked residues | Rattus sp. | alpha-D-mannopyranose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 110000, reducing and non-reducing SDS-PAGE | Rattus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | 6.5 | - |
Rattus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | 0.6 | swainsonine | - |
Rattus sp. |