Cloned (Comment) | Organism |
---|---|
expressed in Halobacterium sp. NRC-1 under the control of a cold shock protein (cspD2) gene promoter. The enzyme is produced at 20fold higher levels compared to Halorubrum lacusprofundi | Halorubrum lacusprofundi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | requires 4-4.5 M NaCl or KCl for maximal activity | Halorubrum lacusprofundi | |
NaCl | requires 4-4.5 M NaCl or KCl for maximal activity | Halorubrum lacusprofundi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78060 | - |
x * 78060, calculated from sequence | Halorubrum lacusprofundi |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 10%, 3 h, 30-35% reduction in activity | Halorubrum lacusprofundi |
isoamyl alcohol | 10%, 3 h, 30-35% reduction in activity | Halorubrum lacusprofundi |
Methanol | 10%, 3 h, 5% reduction in activity | Halorubrum lacusprofundi |
n-Butanol | 10%, 3 h, 30-35% reduction in activity | Halorubrum lacusprofundi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halorubrum lacusprofundi | B9LW38 | - |
- |
Halorubrum lacusprofundi DSM 5036 | B9LW38 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Halorubrum lacusprofundi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
110.8 | - |
25°C, pH 6.5, substrate: 2-nitrophenyl beta-D-galactopyranoside | Halorubrum lacusprofundi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Halorubrum lacusprofundi | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Halorubrum lacusprofundi DSM 5036 | 2-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78060, calculated from sequence | Halorubrum lacusprofundi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in 4 M KCl and at pH 6.5 | Halorubrum lacusprofundi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
10% of maximaln activity | Halorubrum lacusprofundi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
substrate: 2-nitrophenyl beta-D-galactopyranoside. Measured at 37°C and in 4 M KCl | Halorubrum lacusprofundi |