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Literature summary for 3.2.1.23 extracted from

  • Karan, R.; Capes, M.D.; DasSarma, P.; DasSarma, S.
    Cloning, overexpression, purification, and characterization of a polyextremophilic beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi (2013), BMC Biotechnol., 2013, 0000.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Halobacterium sp. NRC-1 under the control of a cold shock protein (cspD2) gene promoter. The enzyme is produced at 20fold higher levels compared to Halorubrum lacusprofundi Halorubrum lacusprofundi

Metals/Ions

Metals/Ions Comment Organism Structure
KCl requires 4-4.5 M NaCl or KCl for maximal activity Halorubrum lacusprofundi
NaCl requires 4-4.5 M NaCl or KCl for maximal activity Halorubrum lacusprofundi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78060
-
 x * 78060, calculated from sequence Halorubrum lacusprofundi

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 10%, 3 h, 30-35% reduction in activity Halorubrum lacusprofundi
isoamyl alcohol 10%, 3 h, 30-35% reduction in activity Halorubrum lacusprofundi
Methanol 10%, 3 h, 5% reduction in activity Halorubrum lacusprofundi
n-Butanol 10%, 3 h, 30-35% reduction in activity Halorubrum lacusprofundi

Organism

Organism UniProt Comment Textmining
Halorubrum lacusprofundi B9LW38
-
-
Halorubrum lacusprofundi DSM 5036 B9LW38
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Halorubrum lacusprofundi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
110.8
-
 25°C, pH 6.5, substrate: 2-nitrophenyl beta-D-galactopyranoside Halorubrum lacusprofundi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Halorubrum lacusprofundi 2-nitrophenol + beta-D-galactose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Halorubrum lacusprofundi DSM 5036 2-nitrophenol + beta-D-galactose
-
 ?

Subunits

Subunits Comment Organism
? x * 78060, calculated from sequence Halorubrum lacusprofundi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 in 4 M KCl and at pH 6.5 Halorubrum lacusprofundi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4
-
 10% of maximaln activity Halorubrum lacusprofundi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 substrate: 2-nitrophenyl beta-D-galactopyranoside. Measured at 37°C and in 4 M KCl Halorubrum lacusprofundi