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Literature summary for 3.2.1.23 extracted from

  • Dabrowski, S.; Maciunska, J.; Synowiecki, J.
    Cloning and nucleotide sequence of the thermostable beta-galactosidase gene from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme (1998), Mol. Biotechnol., 10, 217-222.
    View publication on PubMed
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus woesei

Organism

Organism UniProt Comment Textmining
Pyrococcus woesei O52629
-
-
Pyrococcus woesei DSM 3773 O52629
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus woesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pyrococcus woesei 2-nitrophenol + beta-D-galactopyranose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pyrococcus woesei DSM 3773 2-nitrophenol + beta-D-galactopyranose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
93
-
-
 Pyrococcus woesei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
93 110 93°C: maximal activity, 110°C: active at temperatures up to Pyrococcus woesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 4 h, 11% loss of activity Pyrococcus woesei
93
-
 4 h, 15% loss of activity Pyrococcus woesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4 6.7 pH 5.4: about 90% of maximal activity, pH 6.7: about 40% of maximal activity Pyrococcus woesei