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Literature summary for 3.2.1.23 extracted from

  • Maischberger, T.; Leitner, E.; Nitisinprasert, S.; Juajun, O.; Yamabhai, M.; Nguyen, T.H.; Haltrich, D.
    beta-galactosidase from Lactobacillus pentosus: purification, characterization and formation of galacto-oligosaccharides (2010), Biotechnol. J., 5, 838-847.
    View publication on PubMed
Search for more literature for 3.2.1.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.67
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 30°C Lactiplantibacillus pentosus
38
-
 lactose pH 6.5, 30°C Lactiplantibacillus pentosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enhances activity and stability Lactiplantibacillus pentosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE, small subunit Lactiplantibacillus pentosus
72000
-
 SDS-PAGE, large subunit Lactiplantibacillus pentosus
105000
-
 SDS-PAGE Lactiplantibacillus pentosus

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus pentosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using (NH4)2SO4 fractionation and affinity chromatography Lactiplantibacillus pentosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
97
-
 pH 6.5, 30°C, substrate: 2-nitrophenyl beta-D-galactopyranoside Lactiplantibacillus pentosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Lactiplantibacillus pentosus 2-nitrophenol + beta-D-galactose
-
 ?
lactose + H2O
-
 Lactiplantibacillus pentosus D-galactose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Lactiplantibacillus pentosus

Synonyms

Synonyms Comment Organism
beta-galactosidase
-
 Lactiplantibacillus pentosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 temperature optimum for 2-nitrophenyl-beta-D-galactopyranoside Lactiplantibacillus pentosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 65 temperature optimum for galactose hydrolysis Lactiplantibacillus pentosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 lactose pH 6.5, 30°C Lactiplantibacillus pentosus
540
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 30°C Lactiplantibacillus pentosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in glycine buffer when using 2-nitrophenyl beta-D-galactopyranoside as substrate Lactiplantibacillus pentosus
8
-
 in glycine buffer when using lactose as substrate Lactiplantibacillus pentosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.53
-
 lactose pH 6.5, 30°C Lactiplantibacillus pentosus
325
-
 2-nitrophenyl beta-D-galactopyranoside pH 6.5, 30°C Lactiplantibacillus pentosus