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Literature summary for 3.2.1.23 extracted from

  • Wang, H.; Luo, H.; Bai, Y.; Wang, Y.; Yang, P.; Shi, P.; Zhang, W.; Fan, Y.; Yao, B.
    An acidophilic beta-galactosidase from Bispora sp. MEY-1 with high lactose hydrolytic activity under simulated gastric conditions (2009), J. Agric. Food Chem., 57, 5535-5541.
    View publication on PubMed
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS115 cells Bispora sp.

General Stability

General Stability Organism
the enzyme is strongly resistant to pepsin and trypsin digestion, after incubation in SGF at 37°C for 60 min recombinant BgalA retains almost all of its activity at the whole stomach stage with or without the presence of pepsin Bispora sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 41.5% residual activity at 10 mM Bispora sp.
EDTA 86.2% residual activity at 10 mM Bispora sp.
Mn2+ 90.1% residual activity at 5 mM Bispora sp.
SDS 7.2% residual activity at 5 mM Bispora sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 lactose in glycine-HCl buffer (pH 1.5) Bispora sp.
5.22
-
 2-nitrophenyl-beta-D-galactopyranoside in glycine-HCl buffer (pH 1.5) Bispora sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 108.7% relative activity at 10 mM Bispora sp.
Co2+ 109.1% relative activity at 10 mM Bispora sp.
Cr3+ 115.0% relative activity at 2 mM Bispora sp.
Cu2+ 110.1% relative activity at 10 mM Bispora sp.
Fe3+ 104.4.4% relative activity at 10 mM Bispora sp.
Hg2+ 110.4% relative activity at 2 mM Bispora sp.
K+ 107.7% relative activity at 10 mM Bispora sp.
Li+ 110.1% relative activity at 10 mM Bispora sp.
additional information not stimulated by Mg2+ and Mn2+ Bispora sp.
Na+ 105.3% relative activity at 2 mM Bispora sp.
Ni2+ 103.4% relative activity at 2 mM Bispora sp.
Pb2+ 105.6% relative activity at 2 mM Bispora sp.
Zn2+ 105.2% relative activity at 10 mM Bispora sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 calculated from amino acid sequence Bispora sp.
130000
-
 gel filtration Bispora sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Bispora sp. under simulated gastric conditions, BgalA has greater stability (about 100%) and hydrolysis ratio (more than 80%) toward milk lactose than the commercially available beta-galactosidase from Aspergillus oryzae (ATCC 20423) D-galactose + D-glucose + oligosaccharide the main products of lactose hydrolysis by recombinant BgalA are 19.5% D-galactose, 72.5% D-glucose, and 8.0% oligosaccharide ?

Organism

Organism UniProt Comment Textmining
Bispora sp. C7SLY9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Bispora sp.

Purification (Commentary)

Purification (Comment) Organism
Q sepharose column chromatography Bispora sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bispora sp. 2-nitrophenol + beta-D-galactose
-
 ?
lactose + H2O under simulated gastric conditions, BgalA has greater stability (about 100%) and hydrolysis ratio (more than 80%) toward milk lactose than the commercially available beta-galactosidase from Aspergillus oryzae (ATCC 20423) Bispora sp. D-galactose + D-glucose + oligosaccharide the main products of lactose hydrolysis by recombinant BgalA are 19.5% D-galactose, 72.5% D-glucose, and 8.0% oligosaccharide ?

Synonyms

Synonyms Comment Organism
BgalA
-
 Bispora sp.
lactase
-
 Bispora sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bispora sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 80 the enzyme retains more than 40% of maximum activity between 37 and 80°C Bispora sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 no activity is lost after incubation at 60°C for 60 min and more than 60% of the initial activity is retained after incubation at 70°C for 60 min, most of the enzymatic activity is lost within 2 min at 80°C Bispora sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5
-
-
 Bispora sp.

pH Range

pH Minimum pH Maximum Comment Organism
0.5 8 purified recombinant BgalA has measurable enzymatic activity over a pH range of 0.5 through 8.0 (measured at 37°C) Bispora sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
0.5 12 the enzyme retains more than 50% of its initial activity after incubation at pH 0.5 through 12.0 for 60 min Bispora sp.