Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.91 | - |
4-nitrophenyl-beta-D-galactopyranoside | at pH 6.5 | Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
SDS-PAGE | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
serotype 2 strain D39 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Streptococcus pneumoniae |
Purification (Comment) | Organism |
---|---|
Imac resin column chromatography | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Streptococcus pneumoniae | 4-nitrophenol + beta-D-galactose | - |
? | |
additional information | the SPD0065 protein shows no activity towards 4-methylumbelliferyl-N-acetyl-alpha-D-glucosaminide, 4-methylumbeliferyl-alpha-D-glucopyranoside, 2-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-D-glucoside 6-phosphate, 4-nitrophenyl-alpha-D-mannose 6-phosphate, 4-nitrophenyl-alpha-D-galactose 6-phosphate, and 4-nitrophenyl-beta-D-glucose 6-phosphate | Streptococcus pneumoniae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SPD_0065 protein | - |
Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptococcus pneumoniae |
Organism | Comment | Expression |
---|---|---|
Streptococcus pneumoniae | the expression of SPD_0065 protein in cells grown in mucin-containing medium or tissues harvested from infected animals is significantly upregulated compared to that in pneumococci from glucose-containing medium | up |