Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Clostridium perfringens |
Protein Variants | Comment | Organism |
---|---|---|
D429A | the mutant has wild type activity | Clostridium perfringens |
D453A | inactive | Clostridium perfringens |
E354A | the activity of the E354A mutant with nonactivated natural substrates is 1100fold lower than that of the wild type enzyme, while its activity is only 10fold lower when assayed with 2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | Clostridium perfringens |
E467A | the mutant has wild type activity | Clostridium perfringens |
E506A | inactive | Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | mutant enzyme E354A, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens | |
0.059 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | mutant enzyme E467A, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens | |
0.061 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | mutant enzyme D429A, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens | |
0.064 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | wild type enzyme, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
x * 88000, SDS-PAGE | Clostridium perfringens |
88150 | - |
subunit, calculated from amino acid sequence | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and MonoQ column chromatography | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside + H2O | - |
Clostridium perfringens | 2,4-dinitrophenol + 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | - |
? | |
Gal-alpha(1,3)-Gal-beta-2,4-dinitrophenyl + H2O | - |
Clostridium perfringens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 88000, SDS-PAGE | Clostridium perfringens |
Synonyms | Comment | Organism |
---|---|---|
EABase | - |
Clostridium perfringens |
endo-beta-galactosidase | - |
Clostridium perfringens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | mutant enzyme E354A, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens | |
1.35 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | mutant enzyme D429A, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens | |
1.55 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | mutant enzyme E467A, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens | |
1.75 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | wild type enzyme, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.33 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | mutant enzyme E354A, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens | |
21.67 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | mutant enzyme D429A, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens | |
26.67 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | mutant enzyme E467A, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens | |
26.67 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside | wild type enzyme, in 20 mM sodium acetate buffer at pH 6.0, 37°C | Clostridium perfringens |