General Stability | Organism |
---|---|
the immobilized enzyme (on Ca-alginate gel beads) loses about 80% of activity after the initial cycle in all cases, the activity decreases gradually in the ensuing cycles, and the residual activity is only around 15% after 3 cycles | Aspergillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sp. | - |
- |
- |
Aspergillus sp. AF | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, free enzyme in phosphate buffer (0.05 M, pH 6.5), 30 days, about 90% loss of activity | Aspergillus sp. |
4°C, immobilized enzyme (on Ca-alginate gel beads) in 0.045 M CaCl2 aqueous solution, 30 days, about 10% loss of activity | Aspergillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus sp. | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus sp. AF | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Aspergillus sp. | D-galactose + D-glucose | - |
? | |
lactose + H2O | - |
Aspergillus sp. AF | D-galactose + D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
free enzyme | Aspergillus sp. |
55 | - |
immobilized enzyme (on Ca-alginate gel beads) | Aspergillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | in the range of 40-60°C, the free and immobilized enzymes are very stable and possess activity of more than 80% of the original values | Aspergillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | when the temperature is higher than 60°C for immobilized enzyme (on Ca-alginate gel beads) and above 50°C for free enzyme, an obvious loss of activity is observed, below 40°C the activity for both forms of enzymes decreases substantially | Aspergillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | the immobilized enzyme (on Ca-alginate gel beads) exhibits broad peak of optimum pH in the acid region between pH 3.0 and pH 6.0, having little difference of activity | Aspergillus sp. |
4 | 5 | free enzyme | Aspergillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.6 | 7 | at pH 2.6 free enzyme loses more than 30% activity after 24 h, while the immobilized enzyme (on Ca-alginate gel beads) retains almost the same activity, at pH 7.0 the free enzyme loses more than 90% activity after 24 h while the immobilized enzyme retains around 40% activity | Aspergillus sp. |