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Literature summary for 3.2.1.23 extracted from

  • Bialkowska, A.M.; Cieslinski, H.; Nowakowska, K.M.; Kur, J.; Turkiewicz, M.
    A new beta-galactosidase with a low temperature optimum isolated from the antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization (2009), Arch. Microbiol., 191, 825-835.
    View publication on PubMed
Search for more literature for 3.2.1.23

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 53% rise in activity in the presence of 10 mM 2-beta-mercaptoethanol Arthrobacter sp.
dithiothreitol 25% rise in activity in the presence of 10 mM dithiothreitol Arthrobacter sp.
additional information the enzyme is not stimulated by glutathione Arthrobacter sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TOP10F‘ cells Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoic acid complete inhibition at 10 mM Arthrobacter sp.
Ca2+ 95% residual activity at 5 mM Arthrobacter sp.
Cu2+ complete inhibition at 5 mM Arthrobacter sp.
Pb2+ 15% residual activity at 5 mM Arthrobacter sp.
Zn2+ 50% residual activity at 5 mM Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 49% increase of activity at 5 mM Arthrobacter sp.
Mn2+ 11% increase of activity at 5 mM Arthrobacter sp.
additional information the enzyme is not affected by Mg2+ Arthrobacter sp.
Na+ 53% increase of activity at 5 mM Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
113695
-
 4 * 113695, calculated from amino acid sequence Arthrobacter sp.
116000
-
 4 * 116000, native and SDS-PAGE Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Arthrobacter sp.
-
 D-galactose + D-glucose
-
 ?
lactose + H2O Arthrobacter sp. 20B
-
 D-galactose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. 20B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, PABTG-Sepharose column chromatography, and Sephacryl S-300 gel filtration Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.35
-
 cell extract, in 50 mM potassium phosphate buffer, pH 7.3, containing 100 mM NaCl and 100 mM beta-mercaptoethanol Arthrobacter sp.
2.6
-
 after 7fold purification, in 50 mM potassium phosphate buffer, pH 7.3, containing 100 mM NaCl and 100 mM beta-mercaptoethanol Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O 3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside Arthrobacter sp. 2-nitrophenol + beta-D-galactose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O 3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside Arthrobacter sp. 20B 2-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside Arthrobacter sp. 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside Arthrobacter sp. 20B 4-nitrophenol + beta-D-galactose
-
 ?
lactose + H2O
-
 Arthrobacter sp. D-galactose + D-glucose
-
 ?
lactose + H2O
-
 Arthrobacter sp. 20B D-galactose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 113695, calculated from amino acid sequence Arthrobacter sp.
homotetramer 4 * 116000, native and SDS-PAGE Arthrobacter sp.

Synonyms

Synonyms Comment Organism
BgaS
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 45 the enzyme displays 52-85% maximum activity at 0-20°C, about 50% activity is seen at 45°C Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 35 the enzyme remains stable from 0-35°C, above 35°C there is a constant drop of activity with about 60% relative activity at 40°C, 30% relative activity at 45°C, and no activity at 60-70°C Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
 Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8 the enzyme remains stable from pH 5.5-8.0, about 40% relative activity at pH 4.0 and 10.0, about 70% relative activity at pH 5.0 and 9.0, less than 10% activity at pH 11.0 Arthrobacter sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5
-
 at 25°C Arthrobacter sp. Zn2+