Literature summary for 3.2.1.23 extracted from

Nichols, E.R.; Craig, D.B.
Single molecule assays reveal differences between in vitro and in vivo synthesized beta-galactosidase (2008), Protein J., 27, 376-383.

Search for more literature for 3.2.1.23

Application

Application	Comment	Organism
biotechnology	single enzyme molecule assays performed on the native enzyme as well as recombinant enzyme with and without His-tag reveal significant differences in the average combined turnover numbers indicating that in vivo and in vitro produced enzymes are not identical and the presence of a C-terminal His-tag may alter the activity of beta-galactosidase	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, with and without His-tag	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	single enzyme molecule assays performed on the native enzyme as well as recombinant enzyme with and without His-tag reveal significant differences in the average combined turnover number indicating that in vivo and in vitro produced enzymes are not identical and the presence of a C-terminal His-tag may alter the activity of beta-galactosidase. Average combined turnover numbers of 35800 per min and 31700 per min are obtained respectively for the in vitro synthesized enzyme from strain 35321 with the absence and presence of a C-terminal His6-tag	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

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Release 2023.1 (January 2023)