Literature summary for 3.2.1.23 extracted from

Putrins, M.; Ilves, H.; Kivisaar, M.; Horak, R.
ColRS two-component system prevents lysis of subpopulation of glucose-grown Pseudomonas putida (2008), Environ. Microbiol., 10, 2886-2893.

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Cloned(Commentary)

Cloned (Comment)	Organism
ColR and oprB1 knockouts are constructed and expressed in Escherichia coli	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	strain PaW85 (isogenic to fully sequenced KT2440)	Pseudomonas putida	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	beta-galactosidase activity is barely detectable in supernatants of wild-type and oprB1-deficient strains, the supernatant of glucose-grown colR-deficient strain contains 4% of total beta-galactosidase activity of the cell suspension.	Pseudomonas putida
additional information	-	Membrane permeability of colRoprB1 double mutant is similar to that of the wild-type regardless of the presence of phenol in the growth medium.	Pseudomonas putida
additional information	-	The oprB1 single mutant also behaves like the wild type in membrane permeability test.	Pseudomonas putida
additional information	-	The presence of phenol in the growth medium of colR mutant increases the proportion of extracellular beta-galactosidase activity up to 15%.	Pseudomonas putida
additional information	-	To evaluate membrane permeability, measurement of the activity of an intracellular enzyme beta-galactosidase in nonpermeabilized cells is done. beta-galactosidase is assayed after the bacterial cell membrane is permeabilized with SDS and chloroform. beta-galactosidase activity of untreated cells is compared with that of permeabilized cells. As a source of beta-galactosidase, a plasmid pKTlacZS/C containing the tnpA promoter of the transposon Tn4652 upstream the lacZ gene is used. beta-galactosidase activity in permeabilized glucose-grown cells of all studied genetic backgrounds is about 80 Miller units.	Pseudomonas putida
additional information	-	Up to 15% of total beta-galactosidase activity is detectable in colR-deficient cells grown on glucose minimal plates, and about 35% when cells are grown on glucose medium supplemented with 1 mM phenol. The membrane of colR-deficient strain is more permeable and the presence of phenol further increases the leakiness of the membrane of colR mutant.	Pseudomonas putida
additional information	-	When cells are assayed without the prior chloroform and SDS treatment, differences between the strains become evident. Only 4% of total beta-galactosidase activity is measurable in nonpermeabilized wild-type cells regardless of the presence of phenol in the growth medium.	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
beta-galactosidase	-	Pseudomonas putida

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Release 2023.1 (January 2023)