Cloned (Comment) | Organism |
---|---|
gene lacS, expression in Saccharomyces cerevisiae strain ScKY117 using a galactose-inducible promoter, the strong, heat-inducible upstream activating RcC9 sequence of the yeast genes GAL1 and GAL10, originally isolated from Caenorhabditis elegans, transcription of the lacS gene under the control of this sequence is rapidly and efficiently induced by heat shock, the recombinant enzyme is identical in molecular mass, thermostability, and thermophilicity to the native enzyme, heat induction by growth at 39°C for 45 min, overview | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
gene lacS | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme partially from Saccharomyces cerevisiae by heat treatment at 75°C for 30 min, and ultrafiltration | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.72 | - |
partially purified enzyme, 37°C | Saccharolobus solfataricus |
16 | - |
partially purified enzyme, 75°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactose | - |
? | |
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 5-bromo-4-chloro-indol + beta-D-galactose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
above | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
partially purified enzyme, 2 h, completely stable | Saccharolobus solfataricus |