Protein Variants | Comment | Organism |
---|---|---|
additional information | modulation of the catalytic properties of the enzyme using different supports and immobilization strategies, e.g. bearing glyoxyl, epoxy, or BrCN groups or by glutaraldehyde crosslinking on matrices containing primary amino groups, different derivatives exhibit very different synthetic activity/hydrolytic activity ratios in transglycosylation reactions strongly depending on the experimental conditions, overview | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Escherichia coli | - |
D-glucose + D-galactose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Escherichia coli | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Escherichia coli | D-glucose + D-galactose | - |
? | |
additional information | the enzyme also exhibits transglycosylation activity with lactose as acceptor | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
transglycosylation assay at | Escherichia coli |
7 | - |
assay at | Escherichia coli |