Any feedback?
Literature summary for 3.2.1.23 extracted from
- Purification of beta-galactosidase from Erythrina indica: involvement of tryptophan in active site (2007), Biochim. Biophys. Acta, 1770, 1506-1512.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-galactose | product inhibition | Erythrina variegata |  |
| diethyldicarbonate | - |
Erythrina variegata | |
| Hg2+ | complete inhibition at 5 mM | Erythrina variegata | |
| additional information | no inhibition by PMSF, DTNB, phenyl glyoxal, N-acetyl imidazole, and N,N'-dicyclohexyl carbodiimide | Erythrina variegata | |
| N-bromosuccimide | strong inhibition, attacks a tryptophan residue | Erythrina variegata | |
| Zn2+ | complete inhibition at 5 mM | Erythrina variegata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 4.6, 30°C | Erythrina variegata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
gel filtration | Erythrina variegata |
| 78000 | - |
SDS-PAGE | Erythrina variegata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Erythrina variegata | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme is less glycosylated than other glycosidases | Erythrina variegata |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 135fold by ammonium sulfate fractionation, concanavalin A affinity chromatography, anion and cation exchange chromatography, and gel filtration to homogeneity | Erythrina variegata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Erythrina variegata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 109 | - |
purified enzyme | Erythrina variegata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Erythrina variegata | 4-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Erythrina variegata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Erythrina variegata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
- |
Erythrina variegata |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
