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Literature summary for 3.2.1.23 extracted from

  • Balasubramaniam, S.; Lee, H.C.; Lazan, H.; Othman, R.; Ali, Z.M.
    Purification and properties of a beta-galactosidase from carambola fruit with significant activity towards cell wall polysaccharides (2005), Phytochemistry, 66, 153-163.
    View publication on PubMed
Search for more literature for 3.2.1.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 nitrophenyl beta-D-galactoside 37°C, pH 4.1,beta-galactosidase IV Averrhoa carambola
3.3
-
 nitrophenyl beta-D-galactoside 37°C, pH 4.1,beta-galactosidase III Averrhoa carambola
4.2
-
 nitrophenyl beta-D-galactoside 37°C, pH 4.1,beta-galactosidase II Averrhoa carambola
21.7
-
 nitrophenyl beta-D-galactoside 37°C, pH 4.1,beta-galactosidase I Averrhoa carambola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 48000 + 1 * 36000, beta-galactosidase I, SDS-PAGE Averrhoa carambola
48000
-
 1 * 48000 + 1 * 36000, beta-galactosidase I, SDS-PAGE Averrhoa carambola
58000
-
 beta-galactosidase III, gel filtration Averrhoa carambola
77000
-
 beta-galactosidase II, gel filtration Averrhoa carambola
84000
-
 beta-galactosidase I, gel filtration Averrhoa carambola
130000
-
 beta-galactosidase IV, gel filtration Averrhoa carambola

Organism

Organism UniProt Comment Textmining
Averrhoa carambola
-
 L. cv. B10
-

Purification (Commentary)

Purification (Comment) Organism
beta-galactosidase I Averrhoa carambola

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Averrhoa carambola
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
332
-
-
 Averrhoa carambola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information beta-galactosidase I is substantially active in hydrolyzing the endogenous wall polymers (1->4)beta-linked spruce galactan at a rate twice as rapid as that against the (1->3)beta-linked gum arabic galactan and (1->6)beta-linked gum arabic galactan Averrhoa carambola ?
-
 ?
nitrophenyl beta-D-galactoside + H2O
-
 Averrhoa carambola nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O beta-galactosidase I Averrhoa carambola o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O beta-galactosidase II Averrhoa carambola o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O beta-galactosidase III Averrhoa carambola o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O beta-galactosidase IV Averrhoa carambola o-nitrophenol + beta-D-galactose
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 48000 + 1 * 36000, beta-galactosidase I, SDS-PAGE Averrhoa carambola

Synonyms

Synonyms Comment Organism
beta-galactosidase I predominant isoform Averrhoa carambola
beta-galactosidase II
-
 Averrhoa carambola
beta-galactosidase III
-
 Averrhoa carambola
beta-galactosidase IV
-
 Averrhoa carambola

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 beta-galactosidase I is unstable at Averrhoa carambola
65
-
 beta-galactosidase II is unstable at Averrhoa carambola
65
-
 beta-galactosidase III is unstable at Averrhoa carambola
65
-
 beta-galactosidase IV is unstable at Averrhoa carambola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4 beta-galactosidase IV Averrhoa carambola
3 4 beta-galactosidase I Averrhoa carambola
3 4 beta-galactosidase II Averrhoa carambola
3 4 beta-galactosidase III Averrhoa carambola

pI Value

Organism Comment pI Value Maximum pI Value
Averrhoa carambola isoelectric focusing beta-galactosidase I
-
 7.2