Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme displays non-Michaelis-Menten reaction kinetics with substrate activation for lactose, which is explained by simultaneous reactions of hydrolysis and transgalactosylation | Thermotoga maritima | |
0.33 | - |
o-nitrophenyl-beta-D-galactoside | 80°C | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | most effective divalent cation for beta-galactosidase activity on both o-nitrophenyl-beta-D-galactoside and lactose | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
129501 | - |
x * 129501, calculated from sequence | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Thermotoga maritima | D-glucose + D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermotoga maritima | o-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 129501, calculated from sequence | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
hydrolysis of lactose | Thermotoga maritima |
85 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 16 h | Thermotoga maritima |
90 | - |
half-life: 16 min | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside and lactose | Thermotoga maritima |