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Literature summary for 3.2.1.23 extracted from

  • Tello-Solis, S.R.; Jimenez-Guzman, J.; Sarabia-Leos, C.; Gomez-Ruiz, L.; Cruz-Guerrero, A.E.; Rodriguez-Serrano, G.M.; Garcia-Garibay, M.
    Determination of the secondary structure of Kluyveromyces lactis beta-galactosidase by circular dichroism and its structure-activity relationship as a function of the pH (2005), J. Agric. Food Chem., 53, 10200-10204.
    View publication on PubMed
Search for more literature for 3.2.1.23

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 according to its CD spectrum, beta-galactosidase is mainly a beta-type protein. The antiparallel beta-sheet appears as the amino type of regular conformation (25%) Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Kluyveromyces lactis
-
 D-glucose + D-galactose
-
 ?

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Kluyveromyces lactis

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Kluyveromyces lactis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1709
-
 purified commercial preparation Kluyveromyces lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces lactis 2-nitrophenol + beta-D-galactose
-
 ?
lactose + H2O
-
 Kluyveromyces lactis D-glucose + D-galactose
-
 ?
additional information structure-activity relationship as a function of the pH, overview Kluyveromyces lactis ?
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Kluyveromyces lactis o-nitrophenol + beta-D-galactose
-
 ?

Subunits

Subunits Comment Organism
More secondary structure by circular dichroism, the enzyme is a beta-type protein, having 22% beta-turns, 14% parallel beta-sheet, 25% antiparallel beta-sheet,34% unordered structure, and only 5% alpha-helix, structure-function relationship, highest secondary structure content at pH 6.5-7.0, overview Kluyveromyces lactis

Synonyms

Synonyms Comment Organism
lactase
-
 Kluyveromyces lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Kluyveromyces lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 structure-activity relationship as a function of the pH, overview Kluyveromyces lactis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5
-
 Kluyveromyces lactis