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Literature summary for 3.2.1.23 extracted from

  • Yoon, J.H.; Mckenzie, D.
    A comparison of the activities of three beta-galactosidases in aqueous-organic solvent mixtures (2005), Enzyme Microb. Technol., 36, 439-446.
No PubMed abstract available
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
o-nitrophenyl-beta-D-galactoside
-
 Aspergillus oryzae
o-nitrophenyl-beta-D-galactoside
-
 Escherichia coli
o-nitrophenyl-beta-D-galactoside
-
 Kluyveromyces marxianus

Organic Solvent Stability

Organic Solvent Comment Organism
2-methyoxyethyl acetate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 70% loss of activity Escherichia coli
2-methyoxyethyl acetate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 70% loss of activity Aspergillus oryzae
Acetone 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity Escherichia coli
Acetone 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae
acetonitrile 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity Escherichia coli
acetonitrile 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 70% loss of activity Aspergillus oryzae
diethyleneglycol diethyl ether 37°C, 50% v/v organic solvent-buffer mixture, 5 min,about 60% loss of activity Escherichia coli
diethyleneglycol diethyl ether 37°C, 50% v/v organic solvent-buffer mixture, 5 min,about 90% loss of activity Aspergillus oryzae
diglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity Escherichia coli
diglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 80% loss of activity Aspergillus oryzae
monoglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 50% loss of activity Escherichia coli
monoglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae
tetraglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 40% loss of activity Escherichia coli
tetraglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae
triethyl phosphate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 40% loss of activity Escherichia coli
triethyl phosphate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae
triglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Escherichia coli
triglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 80% loss of activity Aspergillus oryzae
trimethyl phosphate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity Escherichia coli
trimethyl phosphate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Escherichia coli
-
-
-
Kluyveromyces marxianus
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
beta-galactosidase activity in 80% v/v buffer tetraglyme after 11 h is 9% of the initial activity of the enzyme in 50 mM imidazole/HCl buffer containing 1 mM MgCl2, 145 mM NaCl and 4 mM dithiothreitol at pH 7.0. The recovered activity of the enzyme, measured after dialysis is approximately 45% of the initial activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
o-nitrophenyl-beta-D-galactoside + H2O no hydrolysis activity is observed in 1,4-dioxane, methylsulphoxide, N,N-dimethylformamide, or pyridine Escherichia coli o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O no hydrolysis activity is observed in 1,4-dioxane, methylsulphoxide, N,N-dimethylformamide, or pyridine Kluyveromyces marxianus o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O no hydrolysis activity is observed in 1,4-dioxane, methylsulphoxide, N,N-dimethylformamide, or pyridine Aspergillus oryzae o-nitrophenol + beta-D-galactose
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.23
-
 o-nitrophenyl-beta-D-galactoside 30°C, in 50 mM imidazole/HCl buffer containing 1 mM MgCl2, 145 mM NaCl and 4 mM dithiothreitol at pH 6.5 Kluyveromyces marxianus
0.28
-
 o-nitrophenyl-beta-D-galactoside 37°C, in 50 mM sodium acetate/acetic acid buffer at pH 4.5 Aspergillus oryzae
0.3
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: 2-methyoxyethyl acetate, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
0.3
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: acetone, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
0.3
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: monoglyme, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
0.3
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: triethyl phosphate, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
0.3
-
 o-nitrophenyl-beta-D-galactoside 37°C, in 50 mM imidazole/HCl buffer containing 1 mM MgCl2, 145 mM NaCl and 4 mM dithiothreitol at pH 7.0 Escherichia coli
0.35
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: triglyme, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
0.4
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: 2-methyoxyethyl acetate, 50% v/v organic solvent-buffer mixture Escherichia coli
0.42
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: acetonitrile, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
0.58
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: diglyme, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
0.59
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: trimethyl phosphate, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
0.61
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: acetone, 50% v/v organic solvent-buffer mixture Escherichia coli
0.81
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: acetonitrile, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
0.83
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: trimethyl phosphate, 50% v/v organic solvent-buffer mixture Escherichia coli
0.87
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: tetraglyme, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
0.97
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: diethyleneglycol diethyl ether, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
0.99
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: tetraglyme, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
1.04
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: monoglyme, 50% v/v organic solvent-buffer mixture Escherichia coli
1.09
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: diglyme, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
1.21
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: acetonitrile, 50% v/v organic solvent-buffer mixture Escherichia coli
1.38
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: acetone, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
1.4
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: trimethyl phosphate, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
1.44
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: diethyleneglycol diethyl ether, 50% v/v organic solvent-buffer mixture Escherichia coli
1.55
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: diethyleneglycol diethyl ether, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
1.64
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: triglyme, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
1.67
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: tetraglyme, 50% v/v organic solvent-buffer mixture Escherichia coli
1.71
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: diglyme, 50% v/v organic solvent-buffer mixture Escherichia coli
1.76
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: monoglyme, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
1.84
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: triethyl phosphate, 50% v/v organic solvent-buffer mixture Escherichia coli
2.07
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: 2-methyoxyethyl acetate, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
2.92
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: triethyl phosphate, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.38
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: triglyme, 50% v/v organic solvent-buffer mixture Escherichia coli