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Literature summary for 3.2.1.23 extracted from
- Purification and characterization of isoforms of beta-galactosidases in mung bean seedlings (2001), Phytochemistry, 57, 349-359.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | 1 mM, 92% inhibition of beta-galactosidase I, 87% inhibition of beta-galactosidase II, 95% inhibition of beta-galactosidase III | Vigna radiata |  |
| Ca2+ | 1 mM, 15% inhibition of beta-galactosidase I, 12% inhibition of beta-galactosidase II, 13% inhibition of beta-galactosidase III | Vigna radiata | |
| Cu2+ | 1 mM, 23% inhibition of beta-galactosidase I, 4% inhibition of beta-galactosidase II, 16% inhibition of beta-galactosidase III | Vigna radiata | |
| D-galactono-1,4-lactone | 12.5 mM, 98% inhibition of beta-galactosidase I, II and III, hydrolysis of p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | |
| D-galactose | 50 mM, 72% inhibition of beta-galactosidase I, 71% inhibition of beta-galactosidase II, 72% inhibition of beta-galactosidase III, hydrolysis of p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | |
| Fe2+ | 1 mM, 15% inhibition of beta-galactosidase I, 12% inhibition of beta-galactosidase II, 13% inhibition of beta-galactosidase III | Vigna radiata | |
| Hg2+ | 1 mM, 95% inhibition of beta-galactosidase I, 95% inhibition of beta-galactosidase II, 91% inhibition of beta-galactosidase III | Vigna radiata | |
| lactose | 50 mM, 28% inhibition of beta-galactosidase I, 26% inhibition of beta-galactosidase II, 27% inhibition of beta-galactosidase III, hydrolysis of p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | |
| SDS | 1 mM, 98% inhibition of beta-galactosidase I, 97% inhibition of beta-galactosidase II, 93% inhibition of beta-galactosidase III | Vigna radiata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
1 * 38000 + 1 * 48000, beta-galactosidase I, II and III, SDS-PAGE | Vigna radiata |
| 45000 | - |
beta-galactosidase V, gel filtration | Vigna radiata |
| 48000 | - |
1 * 38000 + 1 * 48000, beta-galactosidase I, II and III, SDS-PAGE | Vigna radiata |
| 73000 | - |
beta-galactosidase IV, gel filtration | Vigna radiata |
| 87000 | - |
beta-galactosidase I, II and III, gel filtration | Vigna radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Vigna radiata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.76 | - |
beta-galactosidase V | Vigna radiata |
| 39.79 | - |
beta-galactosidase I | Vigna radiata |
| 95.49 | - |
beta-galactosidase II | Vigna radiata |
| 95.69 | - |
beta-galactosidase III | Vigna radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | beta-galactosidase I: 11.3% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 4.9% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 8.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 6.8% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | 4-nitrophenol + D-glucose | - |
? | |
| additional information | beta-galactosidase II and II are capable of releasing D-galactose residues from the hemicellulose fraction isolated from mung bean | Vigna radiata | ? | - |
? | |
| p-nitrophenyl alpha-D-mannopyranoside + H2O | beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 49.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + alpha-D-mannose | - |
? | |
| p-nitrophenyl alpha-L-arabinopyranoside + H2O | beta-galactosidase I: 20.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 32.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 24.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 12.5% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 18.5% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + alpha-L-arabinopyranose | - |
? | |
| p-nitrophenyl alpha-L-fucopyranoside + H2O | beta-galactosidase I: 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.6% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + alpha-L-fucose | - |
? | |
| p-nitrophenyl alpha-L-galactopyranoside + H2O | beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 3.9% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + alpha-L-galactose | - |
? | |
| p-nitrophenyl beta-D-fucopyranoside + H2O | beta-galactosidase I: 27.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 35.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 31.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 14.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 26.2% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + beta-D-fucose | - |
? | |
| p-nitrophenyl beta-D-galactopyranoside + H2O | - |
Vigna radiata | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl beta-D-glucopyranoside + H2O | beta-galactosidase I: 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 3.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.6% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + beta-D-glucose | - |
? | |
| p-nitrophenyl beta-D-glucuronide + H2O | beta-galactosidase I: 4.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 15.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 64% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + ? | - |
? | |
| p-nitrophenyl beta-D-mannopyranoside + H2O | beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + beta-D-mannose | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | beta-galactosidase I: 1.5% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 2.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 2.3% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + beta-D-xylose | - |
? | |
| p-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 4.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 10.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside | Vigna radiata | p-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 38000 + 1 * 48000, beta-galactosidase I, II and III, SDS-PAGE | Vigna radiata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
beta-galactosidase I | Vigna radiata |
| 52 | - |
beta-galactosidase II | Vigna radiata |
| 53 | - |
beta-galactosidase III | Vigna radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
beta-galactosidase I, II and III | Vigna radiata |
| 3.8 | - |
beta-galactosidase V | Vigna radiata |
| 4 | - |
beta-galactosidase IV | Vigna radiata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | stable, beta-galactosidase I, II and III | Vigna radiata |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.085 | - |
D-galactono-1,4-lactone | beta-galactosidase II | Vigna radiata | |
| 0.13 | - |
D-galactono-1,4-lactone | beta-galactosidase I | Vigna radiata | |
| 0.17 | - |
D-galactono-1,4-lactone | beta-galactosidase III | Vigna radiata | |
| 11.4 | - |
D-galactose | beta-galactosidase I | Vigna radiata | |
| 13 | - |
D-galactose | beta-galactosidase III | Vigna radiata | |
| 15 | - |
D-galactose | beta-galactosidase II | Vigna radiata | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Vigna radiata | beta-galactosidase I | - |
6.9 |
| Vigna radiata | beta-galactosidase III | - |
7.1 |
| Vigna radiata | beta-galactosidase II | - |
7.5 |
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