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Literature summary for 3.2.1.23 extracted from

  • Cho, Y.J.; Shin, H.J.; Bucke, C.
    Purification and biochemical properties of a galactooligosaccharide producing beta-galactosidase from Bullera singularis (2003), Biotechnol. Lett., 25, 2107-2111.
    View publication on PubMed
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 10 mM, 87% inhibition Hamamotoa singularis
SDS 10 mM, 84% inhibition Hamamotoa singularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
 lactose
-
 Hamamotoa singularis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
-
 Hamamotoa singularis

Organism

Organism UniProt Comment Textmining
Hamamotoa singularis
-
 KCTC 7534
-
Hamamotoa singularis KCTC 7534
-
 KCTC 7534
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hamamotoa singularis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
56
-
-
 Hamamotoa singularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O high transgalactosylation activity Hamamotoa singularis galactooligosaccharides
-
 ?
lactose + H2O high transgalactosylation activity Hamamotoa singularis KCTC 7534 galactooligosaccharides
-
 ?
additional information high transgalactosylation activity resulting in the oligosaccharide conversion of over 34% using pure lactose and cheese whey permeate as substrates Hamamotoa singularis ?
-
 ?
additional information high transgalactosylation activity resulting in the oligosaccharide conversion of over 34% using pure lactose and cheese whey permeate as substrates Hamamotoa singularis KCTC 7534 ?
-
 ?
p-nitrophenyl beta-D-galactopyranoside + H2O
-
 Hamamotoa singularis p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl beta-D-galactopyranoside + H2O
-
 Hamamotoa singularis KCTC 7534 p-nitrophenol + beta-D-galactose
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Hamamotoa singularis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 hydrolytic activity Hamamotoa singularis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 40°C: about 45% of maximal activity, 65°C: about 40% of maximal activity Hamamotoa singularis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 2 h, stable up to Hamamotoa singularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 hydrolytic activity Hamamotoa singularis