Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates | Thermus aquaticus | |
Cys | activates | Thermus aquaticus | |
dithiothreitol | activates | Thermus aquaticus | |
glutathione | activates | Thermus aquaticus | |
Urea | slight activation at 1 mM and at 10 mM | Thermus aquaticus |
General Stability | Organism |
---|---|
thermostability is enhanced by CaCl2 | Thermus aquaticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2.5 mM CoCl2, inhibits hydrolysis of lactose and transferase activity | Thermus aquaticus | |
EDTA | 1 mM, 10% inhibition | Thermus aquaticus | |
iodoacetate | - |
Thermus aquaticus | |
p-chlorobenzene sulfonic acid | - |
Thermus aquaticus | |
p-hydroxymercuribenzoate | - |
Thermus aquaticus | |
phenylmethylsulfonyl fluoride | 1 mM, 23% inhibition | Thermus aquaticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.59 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermus aquaticus | |
36 | - |
lactose | calculated from glucose liberated from lactose | Thermus aquaticus | |
60 | - |
lactose | calculated from disappearance from lactose | Thermus aquaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Thermus aquaticus | |
Co2+ | activates | Thermus aquaticus | |
Fe3+ | activates | Thermus aquaticus | |
K+ | activates | Thermus aquaticus | |
Li+ | activates | Thermus aquaticus | |
Mg2+ | activates | Thermus aquaticus | |
Na+ | activates | Thermus aquaticus | |
Zn2+ | activates | Thermus aquaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 59000, SDS-PAGE | Thermus aquaticus |
700000 | - |
gel filtration | Thermus aquaticus |
750000 | - |
non-denaturing PAGE | Thermus aquaticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | - |
- |
- |
Thermus aquaticus YT-I | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus aquaticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.733 | - |
- |
Thermus aquaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl-beta-D-galactoside + H2O | - |
Thermus aquaticus | beta-D-galactose + 1-naphthol | - |
? | |
1-naphthyl-beta-D-galactoside + H2O | - |
Thermus aquaticus YT-I | beta-D-galactose + 1-naphthol | - |
? | |
1-naphthyl-beta-D-glucoside + H2O | - |
Thermus aquaticus | beta-D-glucose + 1-naphthol | - |
? | |
1-naphthyl-beta-D-glucoside + H2O | - |
Thermus aquaticus YT-I | beta-D-glucose + 1-naphthol | - |
? | |
lactose + arabinose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
lactose + cellobiose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
lactose + fructose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
lactose + fructose | transgalactosylation | Thermus aquaticus YT-I | ? | - |
? | |
lactose + lactulose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
lactose + lyxose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
lactose + rhamnose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
lactose + xylose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
additional information | specific for beta-D-anomeric linkages | Thermus aquaticus | ? | - |
? | |
additional information | specific for beta-D-anomeric linkages | Thermus aquaticus YT-I | ? | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus aquaticus | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus aquaticus YT-I | o-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, SDS-PAGE | Thermus aquaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
at pH 7.0 | Thermus aquaticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 85 | 60°C: about 75% of maximal activity, 85°C: about 90% of maximal activity | Thermus aquaticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostability is enhanced by CaCl2 | Thermus aquaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
at 70°C | Thermus aquaticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | about 45% of maximal activity at pH 4.5 and at pH 6.5 | Thermus aquaticus |