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Literature summary for 3.2.1.23 extracted from

  • Berger, J.L.; Lee, B.H.; Lacroix, C.
    Purification, properties and characterization of a high-molecular-mass beta-galactosidase isoenzyme from Thermus aquaticus (1997), Biotechnol. Appl. Biochem., 25, 29-41.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates Thermus aquaticus
Cys activates Thermus aquaticus
dithiothreitol activates Thermus aquaticus
glutathione activates Thermus aquaticus
Urea slight activation at 1 mM and at 10 mM Thermus aquaticus

General Stability

General Stability Organism
thermostability is enhanced by CaCl2 Thermus aquaticus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 2.5 mM CoCl2, inhibits hydrolysis of lactose and transferase activity Thermus aquaticus
EDTA 1 mM, 10% inhibition Thermus aquaticus
iodoacetate
-
Thermus aquaticus
p-chlorobenzene sulfonic acid
-
Thermus aquaticus
p-hydroxymercuribenzoate
-
Thermus aquaticus
phenylmethylsulfonyl fluoride 1 mM, 23% inhibition Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.59
-
o-nitrophenyl-beta-D-galactoside
-
Thermus aquaticus
36
-
lactose calculated from glucose liberated from lactose Thermus aquaticus
60
-
lactose calculated from disappearance from lactose Thermus aquaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Thermus aquaticus
Co2+ activates Thermus aquaticus
Fe3+ activates Thermus aquaticus
K+ activates Thermus aquaticus
Li+ activates Thermus aquaticus
Mg2+ activates Thermus aquaticus
Na+ activates Thermus aquaticus
Zn2+ activates Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
x * 59000, SDS-PAGE Thermus aquaticus
700000
-
gel filtration Thermus aquaticus
750000
-
non-denaturing PAGE Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-
Thermus aquaticus YT-I
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus aquaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.733
-
-
Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl-beta-D-galactoside + H2O
-
Thermus aquaticus beta-D-galactose + 1-naphthol
-
?
1-naphthyl-beta-D-galactoside + H2O
-
Thermus aquaticus YT-I beta-D-galactose + 1-naphthol
-
?
1-naphthyl-beta-D-glucoside + H2O
-
Thermus aquaticus beta-D-glucose + 1-naphthol
-
?
1-naphthyl-beta-D-glucoside + H2O
-
Thermus aquaticus YT-I beta-D-glucose + 1-naphthol
-
?
lactose + arabinose transgalactosylation Thermus aquaticus ?
-
?
lactose + cellobiose transgalactosylation Thermus aquaticus ?
-
?
lactose + fructose transgalactosylation Thermus aquaticus ?
-
?
lactose + fructose transgalactosylation Thermus aquaticus YT-I ?
-
?
lactose + lactulose transgalactosylation Thermus aquaticus ?
-
?
lactose + lyxose transgalactosylation Thermus aquaticus ?
-
?
lactose + rhamnose transgalactosylation Thermus aquaticus ?
-
?
lactose + xylose transgalactosylation Thermus aquaticus ?
-
?
additional information specific for beta-D-anomeric linkages Thermus aquaticus ?
-
?
additional information specific for beta-D-anomeric linkages Thermus aquaticus YT-I ?
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Thermus aquaticus o-nitrophenol + beta-D-galactose
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Thermus aquaticus YT-I o-nitrophenol + beta-D-galactose
-
?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Thermus aquaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
at pH 7.0 Thermus aquaticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 85 60°C: about 75% of maximal activity, 85°C: about 90% of maximal activity Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermostability is enhanced by CaCl2 Thermus aquaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
at 70°C Thermus aquaticus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 about 45% of maximal activity at pH 4.5 and at pH 6.5 Thermus aquaticus