Application | Comment | Organism |
---|---|---|
nutrition | potential use of the enzyme in the hydrolysis of whey and of milk lactose | Rhizomucor sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Rhizomucor sp. | |
D-galactose | - |
Rhizomucor sp. | |
Hg2+ | - |
Rhizomucor sp. | |
isopropyl thiogalactoside | - |
Rhizomucor sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
p-nitrophenyl beta-D-galactopyranoside | - |
Rhizomucor sp. | |
1.32 | - |
o-nitrophenyl-beta-D-galactoside | - |
Rhizomucor sp. | |
50 | - |
lactose | - |
Rhizomucor sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rhizomucor sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
2 * 120000, SDS-PAGE | Rhizomucor sp. |
250000 | - |
gel filtration | Rhizomucor sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 8% neutral sugar | Rhizomucor sp. |
Purification (Comment) | Organism |
---|---|
- |
Rhizomucor sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200 | - |
- |
Rhizomucor sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Rhizomucor sp. | D-glucose + D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Rhizomucor sp. | o-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Rhizomucor sp. | p-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 120000, SDS-PAGE | Rhizomucor sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Rhizomucor sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
4 h, stable | Rhizomucor sp. |
70 | - |
half-life: 150 min | Rhizomucor sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Rhizomucor sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7.5 | stable | Rhizomucor sp. |