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Literature summary for 3.2.1.23 extracted from

  • Shaikh, S.A.; Khire, J.M.; Khan, K.M.I.
    Characterization of a thermostable extracellular beta-galactosidase from a thermophilic fungus Rhizomucor sp. (1999), Biochim. Biophys. Acta, 1472, 314-322.
    View publication on PubMed

Application

Application Comment Organism
nutrition potential use of the enzyme in the hydrolysis of whey and of milk lactose Rhizomucor sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Rhizomucor sp.
D-galactose
-
Rhizomucor sp.
Hg2+
-
Rhizomucor sp.
isopropyl thiogalactoside
-
Rhizomucor sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.66
-
p-nitrophenyl beta-D-galactopyranoside
-
Rhizomucor sp.
1.32
-
o-nitrophenyl-beta-D-galactoside
-
Rhizomucor sp.
50
-
lactose
-
Rhizomucor sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Rhizomucor sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
2 * 120000, SDS-PAGE Rhizomucor sp.
250000
-
gel filtration Rhizomucor sp.

Organism

Organism UniProt Comment Textmining
Rhizomucor sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 8% neutral sugar Rhizomucor sp.

Purification (Commentary)

Purification (Comment) Organism
-
Rhizomucor sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
-
Rhizomucor sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
Rhizomucor sp. D-glucose + D-galactose
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Rhizomucor sp. o-nitrophenol + beta-D-galactose
-
?
p-nitrophenyl-beta-D-galactoside + H2O
-
Rhizomucor sp. p-nitrophenol + beta-D-galactose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 120000, SDS-PAGE Rhizomucor sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Rhizomucor sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
4 h, stable Rhizomucor sp.
70
-
half-life: 150 min Rhizomucor sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Rhizomucor sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7.5 stable Rhizomucor sp.