Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Thermus sp. | |
Cys | activates | Thermus sp. | |
dithiothreitol | activates | Thermus sp. |
Cloned (Comment) | Organism |
---|---|
- |
Thermus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 10 mM, 32% inhibition | Thermus sp. | |
Fe2+ | 10 mM, 16% inhibition | Thermus sp. | |
PCMB | - |
Thermus sp. | |
phenylmethylsulfonyl fluoride | - |
Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermus sp. | |
19 | - |
lactose | - |
Thermus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, activates | Thermus sp. | |
Mn2+ | 10 mM, activates | Thermus sp. | |
Zn2+ | 10 mM, activates | Thermus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72741 | - |
x * 72741, calculation from nucleotide sequence | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Thermus sp. A4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48.6 | - |
- |
Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Thermus sp. | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Thermus sp. A4 | D-glucose + D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus sp. | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus sp. A4 | o-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72741, calculation from nucleotide sequence | Thermus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 h, stable | Thermus sp. |
85 | - |
2 h, retains more than 75% of its activity | Thermus sp. |
90 | - |
half-life: 1 h | Thermus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.5 | - |
lactose | - |
Thermus sp. | |
66.7 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 30% of maximal activity, pH 8.5: about 50% of maximal activity | Thermus sp. |