Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Aspergillus oryzae | |
Cu2+ | - |
Aspergillus oryzae | |
D-galactose | 0.1 mM, slight inhibition of hydrolysis of o-nitrophenyl-beta-D-galactoside | Aspergillus oryzae | |
Hg2+ | - |
Aspergillus oryzae | |
maltose | 0.1 mM, slight inhibition; of hydrolysis of o-nitrophenyl-beta-D-galactoside | Aspergillus oryzae | |
N-bromosuccinimide | - |
Aspergillus oryzae | |
SDS | - |
Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.72 | - |
o-nitrophenyl-beta-D-galactoside | - |
Aspergillus oryzae | |
18 | - |
lactose | - |
Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
gel filtration, sucrose density gradient centrifugation | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
87.5 | - |
- |
Aspergillus oryzae |
Storage Stability | Organism |
---|---|
2°C, stable for several months | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Aspergillus oryzae | D-glucose + D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Aspergillus oryzae | o-nitrophenol + beta-D-galactose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
- |
Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 35% of maximal activity, 60°C: about 30% of maximal activity | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 h, pH 4.0-9.0, stable, more than 60% loss of activity after 10 min at pH 3.5 | Aspergillus oryzae |
40 | - |
pH 4.5 or pH 6.0, 10 min, stable | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | 30°C, 1 h, stable | Aspergillus oryzae |
4.5 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside | Aspergillus oryzae |
4.8 | - |
hydrolysis of lactose | Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6 | pH 2.5: about 50% of maximal activity, pH 6.0: about 45% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside | Aspergillus oryzae |
3 | 6.5 | pH 3.0: about 45% of maximal activity, pH 6.5: about 40% of maximal activity, hydrolysis of lactose | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
30°C, 10 min, more than 60% loss of activity | Aspergillus oryzae |
4.5 | - |
40°C, 10 min, stable | Aspergillus oryzae |
6 | - |
40°C, 10 min, stable | Aspergillus oryzae |